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中文摘要：
目的探讨柴胡皂甙d（SSd）抗小鼠肺纤维化的作用及其机制。方法180只小鼠按随机数字表法分5组。其中4组为博来霉素（BLM）诱导肺纤维化模型组（气管内注射BLM5mg／kg），分为单纯造模组（BLM组）、地塞米松（DXM）干预组（DXM组）、SSd干预组（SSd组）和SSd＋DXM联合干预组（SSd＋DXM组），每组40只；造模后1h，后3组小鼠分别腹腔注射DXM5mg／kg（0．1ml）、SSd1．8mg／kg（0．l8m1）、SSd＋DXM（0．1ml＋0．18m1），BLM组腹腔注射0．28ml生理盐水，每天1次，连续28d。另1组为正常对照组（NC组，20只），同时间按同样方法注入0．28ml生理盐水。给药第3、7、14、28天每组各处死5只小鼠，取肺组织行常规组织病理学检查，检测肺组织中羟基脯氨酸（HYP）、超氧化物歧化酶（SOD）和丙二醛（MDA）含量；取外周血检测SOD和MDA含量。造模的4组每组各20只小鼠用于观察自然生存状况。结果SSd可明显提高肺纤维化小鼠存活率（SSd组和SSd＋DXM组存活率均为80．0％，BLM组为50．0％，P〈0．05），并显著减轻小鼠肺泡炎和肺纤维化程度。DXM组、SSd组和DXM＋SSd组肺组织HYP含量给药第14、28天明显低于BLM组（P〈0．05），血清及肺组织MDA含量给药第3、7、14天明显低于BLM组（P〈0．05，P〈0．01），血清SOD含量给药第3、7、14天、肺组织SOD含量给药第3、7天明显高于BLM组（P〈0．05，P〈0．01）。结论SSd有很明显的抗肺纤维化作用，其作用机制可能与抗脂质过氧化作用有关。
英文摘要：
Objective To study the therapeutic effects and mechanism of saikosaponin-d （SSd） in mice with bleomycin （BLM）-induced pulmonary fibrosis. Methods According to the random number table, 180 mice were randomly divided into 5 groups. Four groups were pulmonary fibrosis models. Fibrosis model mice were established by intratracheal injection of bleomycin （5 mg~ kg-1 ）. They were BLM, DXM, SSd and SSd ＋ DXM groups （ n = 40 each）. At 1 hour post-modeling, DXM, SSd and SSd ＋ DXM groups were injected ip with dexamethasone （DXM,5 mg ~ kg-~ ~ d-1, 0. 1 ml）, SSd （1.8 mg ~ l~g-1 ~ d-1, 0. 18 ml） ,DXM ＋ SSd （0. 28 nal） respectively qd until Day 28. BLM group was similarly dosed with normal saline. In addition, a normal control group （ NC group, n = 20） treated likewise. The mice were anesthetized and sacrificed at Days 3, 7, 14, 28 for harvests of serum and lung tissue samples. The conventional histopathological changes of lung tissue were observed. Except for NC group, modeling groups of mice were used to observe the natural survival rate. Such indices as superoxide dismutase （SOD） and malonaldehyde （MDA） were examined both in lung tissue and serum samples. And hydroxyproline （HYP） was tested only in lung tissue. Results SSd could markedly increase the survival rate （ 80. 0% in SSd and SSd ＋ DXM groups vs 50. 0% in BLM group, P 〈 0. 05 ） and reduce alveolitis and fibrosis in mice. In comparison with BLM group, the levels of HYP of three treatment groups （ DXM, SSd and SSd ＋ DXM ） in lung tissue was significantly lower （P 〈0. 05） at Days 14 and 28. The levels of MDA both in serum and lung tissue were significantly lower at Days 3, 7 and 14 （ P 〈 0. 05 ）. The serum level of SOD was significantly higher at Days 3, 7 and 14 while the level of SOD in lung tissue was significantly higher at Days 3 and 7 （ P 〈 0. 05, P 〈0.01） mice. Conclusions SSd has marked therapeutic effects upon bleomycin-induced pulmonary fibrosis in And the mechanism may be asso
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