中文摘要：

目的探讨饮水摄入三氯乙烯（trichloroethylene，TCE）对小鼠Th17细胞的影响。方法将48只6周龄雌性BALB／c小鼠按随机数字表法分为空白对照组、溶剂对照组、2．5mg／ml TCE组和5．0mg／ml TCE组，每组12只，染毒组经饮水摄入TCE；每组分别于暴露第14、28、56、84天各处死3只小鼠，取其脾脏及外周血，流式细胞术检测CD4＋T细胞中Th17细胞比例，实时荧光定量PCR测定维甲酸相关孤儿核受体（retinoid—related orphan receptors，RORTγt）mRNA表达水平；ELISA法测定外周血血清白细胞介素-17（IL-17）、白细胞介素-6（IL-6）和转化生长因子-β（TGF—β）含量。结果2．5、5．0mg／ml TCE染毒组小鼠的脾脏CD4＋T细胞中Th17细胞比例第14天时分别为（3．46±0．32）％和（5．45±0．45）％，第84天时分别为（3．47±0．33）％和（4．10±0．39）％，均较同期溶剂对照组[分别为（2．15±0．20）％、（2．16±0．35）％]升高且差异有统计学意义（P值均〈0．01）；其RORγt表达水平第14天时分别为1．870±0．084和1．965±0．060，第56天时分别为1．998±0．079和2．028±0．073，均较同期溶剂对照组（1．77±0．04、1．75±0．09）上调且差异有统计学意义（P值均〈0．05）；其血清IL-17含量第14天时分别为（32．28±5．38）和（34．47±5．02）pg／ml，第28天时分别为（34．87±5．48）和（41．94±6．19）pg／ml，均较同期溶剂对照组升高[分别为（21．57±5．23）、（22．11±5．11）pg／ml]且差异有统计学意义（P值均〈0．05）；其血清IL-6含量第14天时分别为（43．07±6．71）和（47．86±8．52）pg／ml，第56天时分别为（41．32±7．04）和（46．74±9．33）ps／ml，均较同期溶剂对照组[分别为（7．56±7．71）和（28．26±7．22）pg／ml]升高且差异有统计学意义（P值均〈0．05）；其血清TGF—β含量第14天时分别为（17．48±3．06）和（18．93±3?

英文摘要：

Objective This study aimed to investigate the effect of trichloroethylene （TCE） intake via drinking water on Th17 cells in mice. Methods Forty eight six weeks old female BALB/c mice were divided into blank control,vehicle control,2. 5 mg/ml TCE and 5.0 mg/ml TCE groups by random number table（ 12 mice each group） , and exposed to TCE by drinking water. On the 14th , 28th, 56th, 84th days, blood were collected and assayed for IL-17 ,IL-6, and TGF-β concentration in serum through ELISA. Animals were killed and spleen biopsies were taken sterilely. The proportion of Tb17 cells among CD4 ＋ T cells and RORγt mRNA expression level in spleen were measured by FCM and real-time PCR. Results In 2. 5 mg/ml TCE and 5.0 mg/ml TCE group mice,Th17 cells/CD4＋ T cells in spleen were （3.46 ±0. 32） % and （5.45 ± 0.45 ） % on day 14 , （3.47 ± 0. 33 ） % and （4. 10 ± 0. 39 ） % on day 84, which were significantly higher than those for solvent control group at the same time point （ （ 2. 15 ± 0. 20 ） % , （ 2. 16 ± 0. 35 ） %, respectively） （P 〈 0. 01 ）. RORTt mRNA expression levels were （ 1. 870 ± 0. 084） and （ 1. 965 ± 0. 060） on 14 day, （1. 998 ±0. 079） and （2. 028 ±0. 073） on day 56, which were also significantly higher than those for solvent control group at the same time point （ 1.77 ± 0. 04 and 1.75 ± 0. 09, respectively） （ P 〈 0. 05 ）. IL-17 concentrations in serum were （32. 28 ±5.38） and （34. 47 ±5.02） pg/ml on day 14, and （34. 87 ± 5.48 ） and （41.94 ± 6. 19） pg/ml on day 28, which were significantly higher than those for solvent control group at the same time point（ （21.57 ±5.23）, （22. 11 ±5. 11 ） pg/ml）. IL-6 concentration in serum were （43.07 ± 6. 71） and （47.86 ±8.52） pg/ml on day14 , （41.32 ±7.04） and （46. 74 ±9. 33） pg/ml on day 56, which were significantly higher than solvent control group at the same time point （ （7. 56 ± 7. 71 ）and （28. 26 ±7.22） pg/ml）. TGF-β concentrat