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Comparison of clonogenic assay with premature chromosome condensation assay in prediction of human cell radiosensitivity

ISSN号：1007-9327
期刊名称：《世界胃肠病学杂志：英文版》
时间：0
分类：R394[医药卫生—医学遗传学;医药卫生—基础医学]
作者机构：[1]Graduate School of Chinese Academy of Sciences, Beijing 100039, China, Institute of Modem Physics,Chinese Academy of Sciences, Lanzhou 730000, Gansu Province,China, [2]Institute of Modern Physics, Chinese ,Academy of Sciences, Lanzhou 730000, Gansu Province, China
相关基金：Supported by the Key Project of National Natural Science Foundation of China, No. 10335050 and the Key Project of Ministry of Science and Technology of China, No. 2003CCB00200

作者： Zhuan-Zi Wang[1], Wen-Jian Li[2], Hong Zhang[2], Jian-She Yang[2], Rong Qiu[2], Xiao Wang[2]

关键词：染色体, 冷凝技术, 克隆技术, 辐射敏感性, Clonogenic radiosensitivity, Non-rejoining G2-chromatid breaks, Correlation

中文摘要：

瞄准：决定非重返的 G2 染色单体裂缝的数字是否能预言人的房间线的放射敏感度。方法：人的卵巢癌房间(HO8910 ) 的房间线，人的肝细胞瘤房间(HepG2 ) 和肝细胞(L02 ) 与剂量的一个范围被照耀并且在照耀以后在 24 h 房间幸存和非重返的 G2 染色单体裂缝估计了两个。房间幸存被殖民地试金记录。非重返的 G2 染色单体裂缝被在照耀以后在 24 h 数非重返的 G2 染色单体裂缝的数字测量，检测了由过早地，染色体压缩了(PCC ) 技术。结果：一条线的, 线形二次的幸存曲线在三根房间线被观察，并且 HepG2 对 gamma 放射最敏感。一个剂量依赖者线性增加在在在所有房间的照耀排队以后，在 24 h 测量的导致放射的非重返的 G2-PCC 裂缝被观察，并且 HepG2 最产生非重返的 G2-PCC 裂缝的正式就职。靠近的关联在 clonogenic 放射敏感度和导致放射的非重返的 G2-PCC 之间被发现的 A 碎(r = 0.923 ) 。而且，为二或超过二剂量杆的幸存错误关联也是重要的。结论：当二或超过二剂量杆被测试时，非重返的 G2 PCC 裂缝的数字为预言正常和肿瘤房间的放射敏感度保持可观的诺言。

英文摘要：

AIM： To determine whether the number of non-rejoining G2-chromatid breaks can predict the radiosensitivity of human cell lines. METHODS： Cell lines of human ovary carcinoma cells （HO8910）, human hepatoma cells （HepG2） and liver cells （L02） were irradiated with a range of doses and assessed both of cell survival and non-rejoining G2-chromatid breaks at 24 h after irradiation. Cell survival was documented by a colony assay. Non-rejoining G2-chromatid breaks were measured by counting the number of non-rejoining G2 chromatid breaks at 24 h after irradiation, detected by the prematurely chromosome condensed （PCC） technique. RESULTS： A linear-quadratic survival curve was observed in three cell lines, and HepG2 was the most sensitive to γ-radiaUon. A dose-dependent linear increase was observed in radiation-induced non-rejoining G2- PCC breaks measured at 24 h after irradiation in all cell lines, and HepG2 was the most susceptible to induction of non-rejoining G2-PCC breaks. A close correlation was found between the clonogenic radiosensitivity and the radiation-induced non-rejoining G2-PCC breaks （r=0.923）. Furthermore, survival-aberration correlations for two or more than two doses lever were also significant. CONCLUSION： The number of non-rejoining G2 PCC breaks holds considerable promise for predicting the radiosensitMty of normal and tumor cells when two or more than two doses lever is tested.

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