中文摘要：

本研究以新疆核桃粕为实验原料，利用亚临界水辅助提取核桃粕蛋白，分别以提取时间、料液比、提取温度、pH值为单因素研究对蛋白提取率的影响。通过单因素试验确定合适的因素水平，以响应面优化分析得到核桃粕蛋白提取的最佳条件。结果表明：核桃粕的最佳提取工艺为pH9.0、料液比1∶25（g/mL）、提取温度133℃，此条件下的蛋白提取率可达到75.01%。氨基酸的结果分析表明：氨基酸种类齐全，谷氨酸、精氨酸、天冬氨酸3种含量最高，共占氨基酸总量的59.7%，必需氨基酸占20.03%。亚临界水提取蛋白的提取率较高，操作方便，适用于核桃粕蛋白提取。

英文摘要：

The subcritical water extraction of walnut meal protein was optimized by response surface methodology.The variables optimized were extraction time,solid-to-solvent ratio,extraction temperature and pH.The response was the extraction yield of protein.One-factor-at-a-time method was used to determine the appropriate levels of independent variables for further optimization by response surface methodology.The results showed that the optimal conditions were as follows:pH,9.0;solid-to-solvent ratio,1:25(g/mL);and temperature,133℃.Under these conditions,the extraction yield was up to75.01%.The results of amino acid analysis indicated that the walnut protein contained almost all kinds of amino acids with glutamic acid,arginine,and aspartate being the predominant amino acids,accounting for59.7%of the total amino acids.Essential amino acids accounted for20.03%.Sub critical water extraction could be considered as convenient and efficient method for the extraction of walnut protein.