中文摘要：

棉花纤维力量主要在第二等的房间墙免职阶段期间被决定纤维素是否被综合。我们获得了 20-25 d 柱子开花期(DPA ) 的 cDNA 从 109 F_2 子孙的纤维并且开发了用 37 不同教材联合经由 cDNA-AFLP 技术介绍的棉花纤维 transcriptome。F_2 人口从在 Gossypium hirsutum 和 Gossyp 之间的一个种间的十字发源，嗯 barbadense。多态的抄本导出的 138 缺席 / 存在碎裂(TDF ) 与从 100 bp 到 722 bp 的尺寸，这被屏蔽(53.62%) 75 在 F_2 人口的父母之间是多态的。定序 75 个抄本表明他们中的 37 个被报导了是棉花纤维 EST。Nineof 75 抄本序列对 7 克隆的棉花纤维基因相应，编码半胱氨酸朊酶， vacuolar H~+-pyrophosphatase， vacuolar H~+-ATPase，催化子单元， arabinogalactan 蛋白质，通常认为的受体蛋白质 kmase PERK1，在反应调节的人的 GIA/RGA-like gibberel 和纤维素 synthase。一些另外的抄本可以在棉花纤维开发代表新基因碎片。令人惊讶地， 75 个抄本中的 46 个被印射到一个单个连接组。transcriptome 组和定序的 TDF 能在棉花纤维开发的功能的 genomsc 研究用作重要资源。

英文摘要：

Cotton fiber strength is mainly determined during the secondary cell wall deposition stage when cellulose is synthesized. We obtained cDNA of 20—25 d post anthesis （DPA） fiber from 109 F2 progeny and developed a cotton fiber transcriptome profiling via cDNA-AFLP technology using 37 different primer combinations. The F2 population originated from an interspecific cross between Gossypium hirsutum and Gossypium barbadense. One hundred and thirty-eight absence/presence polymorphic transcriptderived fragments （TDFs）, with sizes ranging from 100 bp to 722 bp, were screened. Of these, 75 （53.62%） were polymorphic between the parents of the F2 population. Sequencing the 75 transcripts revealed that 37 of them had been reported to be cotton fiber ESTs. Nine of 75 transcript sequences were homologous to 7 cloned cotton fiber genes, encoding cysteine proteinase, vacuolar H^＋-pyrophosphatase, vacuolar H^＋ -ATPase, catalytic subunit, arabinogalactan protein, putative receptor protein kinase PERK1, GIA/RGA-like gibberellin response modulator and cellulose synthase. Some other transcripts may represent new gene fragments in cotton fiber development. Surprisingly, 46 of the 75 transcripts were mapped to a single linkage group. The transcriptome groups and the sequenced TDFs could serve as important resources in the functional genomic research of cotton fiber development.