随着糖尿病的发病率不断攀升,有必要建立简单易行的胰岛素的分析方法。胰岛素制剂的纯度检验,胰岛素生产及纯化过程的监控,生物体液及胰岛细胞分泌胰岛素堂登量,重组胰岛素结构的测定及活性研究等都离不开胰岛素的测定。而胰岛素的色谱分析是一个年轻的领域。本文按照体内外样品、检测方法和样品制备总结了近年胰岛素的色谱分析方法。
It is necessary to estabilish simple and flexible methods for assaying insulin owing to increasing incidence of diabetes mellitus worldwide. Monitoring insulin in procedure of produclion and purification, determining insulin content from bio-tissue fluid and single islets of Langerhans, and studying insulin molecular structure are all dependent on chromatographic analysis. The chromatographic analysis of insulin is a new field, The review summarized all kinds of chromatographic method for insulin according to the source of insulin samples,the method used,and the pretreatment of insulin.