中文摘要：

应用反转录（RT-P（1R）技术从河南传染性法式囊发病鸡中总砌姐克隆出1461bp的vP2基因，并将其克隆于pGEM-T载体，筛选并构建了pT-VP2克隆载体。序列分析表明，该VP2基因及推导氨基酸序列与日本和欧洲超强毒株序列有较高的同源性，特征性氨基酸变异相似，进化分析也表明Xin-1毒株与欧洲超强毒株UK661和日本超强毒株0KYM位于同一进化分支，提示Xin-1株病毒可能为超强毒株。

英文摘要：

Abstract：The VP2 gene about 1461bp was obtained from the total RNA of the chicken IBDV by RT-PCR, then the VP2 gene was cloned into the vector pGEM T to construct vector pT- VP2. sequence analysis showed that the high homology of target gene and animo acid with the wlBDV from Japan and Europe. The similar variation of characteristic amino acid implied strain Xin 1 was probably vvIBDV. Phylogenetic analysis showed Xin - 1 was in the same lineage to vvIBDV UK661 and OKYM isolated from Europe and Japan respectively, implying Xin- 1 might be vvIBDV.