中文摘要：

目的：探索小鼠皮下和内脏前脂肪细胞的培养方法。方法：取C57BL／6J小鼠腹股沟皮下脂肪和附睾旁内脏脂肪组织，采用胶原酶消化过滤法获得梭形细胞，对培养的细胞进行形态学观察，诱导分化后用油红O染色法染色定性，荧光定量PCR检测成脂功能标志基因表达情况。结果：培养出的梭形细胞成分均一，增殖旺盛，诱导后分化率高，经油红O染色证实分离获得的前脂肪细胞可以分化为成熟脂肪细胞，荧光定量PCR检测成脂功能标志基因Fabp4（fattyacidbindingprotein4）的表达量明显升高。结论：从C57BL／6J小鼠腹股沟皮下和附睾旁内脏脂肪组织中可以分离出具有很强增殖、分化能力的皮下和内脏前脂肪细胞．这种前脂肪细胞原代培养模型的建立为在体外进一步研究皮下、内脏脂肪功能的差异提供了良好的基础。

英文摘要：

Objective：To establish a culture method for primary mouse subcutaneous and visceral adipocyte precursor cells. Methods：Fibroblast-like cells were collected from C57BL/6J mice inguinal and epididymal adipose tissue. The morphological changes of the cultured cells were observed,the intracytoplasmic lipid of the culture cells was determined using oil red O staining, and the expression of the specific adipogenic gene was determined by real time PCR. Results：The cultured fibroblast-like cells showed highly homogeneous appearance with active proliferation and differentiate into mature adipocytes. Oil red O staining, morphological observation,and expression of specific adipogenic gene Fabp4 verified these cells as adipocyte. Conclusion：Adipocyte precursor cells are presented in C57BL/6J mice inguinal and epididymal adipose tissue and possess the potential to proliferate and differentiate into mature adipocyte. The establishment of primary culture of mouse subcutaneous and visceral adipocyte precursor cells is important for further studies of the differences between subcutaneous and visceral adipocyte in vitro.