为了研究并优化辣蓼总黄酮的提取工艺,采用6种不同提取方法提取辣蓼总黄酮,以总黄酮含量为考察指标,筛选出最佳的提取方法;采用正交试验设计,对最佳提取方法中的醇沉乙醇终浓度、料液比、超声温度3种因素进行优化。结果表明,在6种提取方法中,超声提取配合酶解法的辣蓼总黄酮得率最高,为3.8%;该方法的最优提取条件为:乙醇浓度60%,料液比1∶30,超声温度60℃。该工艺操作简便,重现性强,能耗低,效率高,节省溶剂,有效降低成本,可用于辣蓼中总黄酮的提取。
The aim of the present study was to develop and optimize the extraction technology of total flavonoids from Polygonum hydropiper. Using total flavonoids content as an evaluation index, the extraction efficiency of six different methods were assessed and compared to screen the optimum extraction method; orthogonal design was employed to optimize the key parameters of ethanol final concentration in ethanol precipitation, ratio of solid to liquid and ultrasonic temperature in the screened optimum extraction method. The results showed that the highest yield (3.8%) of total flavonoids was obtained in enzymolysis-uhrasonic coupling method; the optimum extraction conditions were: ethanol concentration 60%, ratio of solid to liquid 1:30, ultrasonic temperature 60 ℃. The developed method has the advantages of simple operation, high reproducibility, low energy consumption, high efficiency, solvent saving and low cost; therefore, it can he used for the extraction of the total flavonoids in Polygonum hydrop ip e rum.