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中文摘要：
以鲢鱼鱼鳞为研究对象，采用酸酶复合法提取鱼鳞胶原蛋白，并对酶法提取工艺中的酶量、时间、料液比等因素进行单因素试验和正交优化。使用氨基酸自动分析仪、聚丙烯酰胺凝胶电泳（SDS—PAGE）和圆二色谱fcD）对酸溶胶原蛋白（ASC）和酶溶胶原蛋白（PSC）的性质进行研究。试验表明，酸提取后的残渣进行酶提取，其最佳工艺条件为：酶量4％，料液比1：25，提取时间60h。ASC提取率达到5．09％，PSC提取率最高达到12．06％，胶原蛋白总提取率为17．15％。氨基酸组成结果表明，ASC和PSC均为典型的I型胶原蛋白；SDS—PAGE电泳分析显示，ASC和PSC的电泳区带与I型胶原蛋白标准品基本一致；CD显示ASC和PSC均保持三股螺旋结构。
英文摘要：
Extraction technology of collagen from silver carp scale was studied by acid-enzyme combined method. Using one-factor-at-a-time combined with orthogonal array design method, the extraction yield of collagen was investigated with respect to enzyme dosage, extraction time and material/liquid ratio in the step of enzymatic treatment. In the present study,the methods of Amino acid analyzer, Sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS-PAGE） and Circular dichroism （CD） have been used to study the properties of acid-soluble collagen（ASC） and pepsin-soluble collagen （PSC）.Then the maximum extraction yield （12.06%） of PSC was obtained at the optimal conditions： enzyme dosage 4% （g/ g）, extraction time 60 h and material/liquid ratio 1：25 （g/mL）. Since the yield of ASC was 5.09%, the extraction yield of collagen was up to17.15%. Amino acid analysis indicates that ASC and PSC were typical I collagen; SDS-PAGE analysis showed that ASC and PSC were similar to those of the standard I collagen; CD indicates the triple helix conformation of ASC and PSC.
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