中文摘要：

背景生物反应器是为在设计学习试图创造能模仿尿膀胱的一个生物反应器的 study.This 的功能的织物产生机械刺激的枢轴的工具机械性质，并且弄平肌肉 cells.Methods 在 urothelial 房间和膀胱上调查一种机械地刺激的文化的效果我们设计了一个生物反应器模仿膀胱的机械性质。一个压力记录系统被用来由在文化房间测量压力评估生物反应器的机械性质。为了在静态、机械地改变的条件下面测试生物反应器， urothelial 房间的生存能力和在生物反应器有教养的光滑的肌肉房间的 biocompatibility，在 7 天的文化以后被测量。在重要房间，尿道的房间和光滑的肌肉房间上评估机械刺激的效果处于模仿的机械条件是有教养的。在那以后，生存能力和房间的分发模式被观察，当维持在房间之间的播种房间的有弹性的膜时，与在非机械的刺激 condition.Results 有教养的房间相比，生物反应器系统成功地产生了类似于打算的规划模型的波形。 urothelial 房间的生存能力之间没有差别(( 91.90 戠瑵猠楴汬氠捡獫搠瑡?牦浯氠牡敧猭浡汰?汣湩捩污琠楲污?湯攠晦捩捡?湡?慳敦祴瀠潲楦敬?桔?楡?景琠楨?瑳摵?慷?潴攠慶畬瑡?桴?晥敦瑣癩湥獥?湡?慳敦祴漠?剏协??椠?档汩牤湥愠敧??潴ㄠ?敹牡?楷桴???湵敤?慮畴慲楬瑳捩挠楬楮慣?敳瑴湩?敍桴摯?桔獩?眭敥?畭瑬?散瑮牥瀬潲灳'虡N敶漬数?慬敢?瑳摵?湥潲汬摥ㄠ????挠楨摬敲?潴漠据?慤汩?剏协????洠??洠?牯??杭?牴慥浴湥?桔?晥敦瑣癩湥獥?敭獡牵獥眠牥?慰敲瑮爭瑡摥?慮瑴湥楴湯愠摮传敶慲瑣癩瑩?楗桴?杧敲獳潩??坏??湯敮獲?伯愠摮

英文摘要：

Background Bioreactors are pivotal tools for generating mechanical stimulation in functional tissue engineering study.This study aimed to create a bioreactor that can simulate urinary bladder mechanical properties, and to investigate the effects of a mechanically stimulated culture on urothelial cells and bladder smooth muscle cells.Methods We designed a bioreactor to simulate the mechanical properties of bladder. A pressure-record system was used to evaluate the mechanical properties of the bioreactor by measuring the pressure in culture chambers. To test the biocompatibility of the bioreactor, viabilities of urothelial cells and smooth muscle cells cultured in the bioreactor under static and mechanically changed conditions were measured after 7-day culture. To evaluate the effect of mechanical stimulations on the vital cells, urethral cells and smooth muscle cells were cultured in the simulated mechanical conditions. After that, the viability and the distribution pattern of the cells were observed and compared with cells cultured in non-mechanical stimulated condition.Results The bioreactor system successfully generated waveforms similar to the intended programmed model while maintaining a cell-seeded elastic membrane between the chambers. There were no differences between viabilities of urothelial cells （（91.90±1.22）% vs. （93.14±1.78）%, P 〉0.05） and bladder smooth muscle cells （（93.41±1.49）% vs.（92.61±1.34）%, P 〉0.05）. The viability of cells and tissue structure observation after cultured in simulated condition showed that mechanical stimulation was the only factor affected cells in the bioreactor and improved the arrangement of cells on silastic membrane.Conclusions This bioreactor can effectively simulate the physiological and mechanical properties of the bladder.Mechanical stimulation is the only factor that affected the viability of cells cultured in the bioreactor. The bioreactor can change the growth behavior of urothelial cells and bladder smooth muscle cells, resulting i