中文摘要：

大约 25,000 根米饭 T-DNA insertional 异种线用有套住倡导者、标注激活的功能的向量 pCAS04 被产生。南部的污点分析表明大约 40% 这些异种是单个拷贝集成，平均 T-DNA 插入数字是 2.28。由这个领域里的广泛的 phenotyping，相当很多个农学的联盟者重要异种被获得。有 4,310 主要异种的组织化学的 GUS 试金表明染色 GUS 频率在花比在各种各样的纸巾和特别高度的以前的报告的高。一些异种的 T-DNA flanking 序列被孤立， T-DNA 插入地点被印射到米饭染色体。flanking 顺序分析表明了正确边阶和左边阶的不同集成模式进米饭染色体。与 Arabidopsis 和白杨相比，它很在米饭在 T-DNA 边阶连接被改变。

英文摘要：

About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,310 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice.