目的:观察运动训练对血液全血和红细胞2,3-二磷酸甘油酸(2,3-DPC-)的影响,并探讨2,3-DPG与运动性贫血之间的关系;方法:国家跆拳道青年队和北京石景山运动技术学校中长跑28名运动员作为研究对象。根据运动员的性别和血红蛋白指标结果分为女性贫血组(n=8)、女性对照组(n=10)、男性贫血组(n=4)和男性对照组(n=6)。应用氰化高铁血红蛋白比色法(HiCN)和紫外测定法用于测定运动员血红蛋白和2,3-DPG水平;结果:运动性贫血男女运动员表现出血红蛋白水平显著低于运动组的特点(女性对照组134.40±6.74rnmol/1,女性贫血组119.75±6.48mmol/l,男性对照组144.00±7.04mmol/l,男性贫血组109.75±11.50mull/l,P〈0.01)。无论是分性别还是不分性别,全血2,3-DPG和红细胞2,3-DPG均未表现出贫血组和对照组的组间显著性差异(P〉0.05);结论:全血和红细胞2,3-DPG的研究结果显示运动性贫血运动员不比正常对照运动员具有更高的2,3-DPG水平,说明从氧分离曲线右移的适应性角度探讨运动性贫血的发生机理是不可行的。
Objective: To observe the changes of whole blood 2,3-diphosphoglycerate and erythrocyte 2,3-diphosphoglycerate after exercise training, and to investigate the relation between 2,3-DPG and sports anemia. Method: 28 members of the Chinese youth team of taekwondo and middle-distance race team of a sport school were the subjects. According to gender and results of hemoglobin (Hb), the subjects were divided into female sports anemia (n= 8), female control ( n = 10 ), male sports anemia ( n = 4 ) and male control ( n = 6 ). Hemiglobincyanide method and UV-test method was used to measure the Hb and 2,3-DPG in blood research samples respectively. Result:there were significant decreases in serum Hb of subjects between sports anemia and control group (female sports anemia group 119.75±6. 48 mmol/l, female control 134.40±6.74 mmol/l,male sports anemia 09. 75±11. 50 retool/l and male control 144.00± 7.04 retool/l, P〈0.01 ). Whether or not considering gender, there were not significant difference between sports anemia group and control group in whole blood 2,3-DPG and erythrocyte 2,3-DPG. Conclusion : Experimental result of 2,3-DPG in whole blood and erythrocyte showed that the 2,3-DPG in sports anemia group was not significantly higher than that of control group. Therefore, it is not possible to study the mechanism of sports anemia based only on the right shift in the oxygen dissociation curve of hemoglobin.