中文摘要：

为探究bta—miR-205和bta—miR-497对牛病毒性腹泻病毒（BVDV）复制的调控作用，在前期建立BVDv感染MDBK细胞miRNAs差异表达谱的基础上，筛选出表达量显著差异的bta—miR-205和bta—miR-497，通过生物信息学软件预测、荧光素酶报告基因检测等方法鉴定bta-miR-205和bta-miR-497的靶基因。应用RT—qPCR和病毒滴度测定等方法验证bta—miR-205和bta—miR-497对BVDV复制的影响。结果发现，bta—miR-205能显著抑制BVDV的复制，而bta—miR-497显著促进BVDV的复制。研究结果为BVD防控提供了新的思路和依据。

英文摘要：

In order to explore the regulation effect of bta-miR-205 and bta-miR-497 on the bovine viral diarrhea virus（BVDV）＇ replication, based on the established miRNAs＇ differential expression profile of BVDV infected MDBK cells, the bta-miR-205 and bta-miR-497 with significantly different expressions were selected. The target genes of bta-miR-205 and bta-miR-497 were identified by the methods of bioinformatics software prediction and luciferase reporter gene verification. The effects of bta-miR-205 and bta-miR-497 on BVDV replication were verified by qRT-PCR and virus titer assay. The results showed that bta-miR-205 significantly inhibited the replication of BVDV, and bta-miR-497 significantly promoted the replication of BVDV. The study provided a new idea and basis for establishing a new technology to control BVDV.