中文摘要：

采用电辅助微生物（电生物）体系对二氯酚（2,4-DCP）进行了还原降解研究,应用聚合酶链式反应-单链构象多肽技术（PCR-SSCP）对微生物群落结构进行解析,并通过16S rRNA基因克隆及测序构建了系统进化树.结果表明,电生物体系对二氯酚的降解符合零级反应动力学特征,体系中主要以肠球菌（Enterococ-cus）、假单胞菌（Pseudomonas）和拟杆菌（Bacteroidales）为优势菌群,这些菌群在电子传递中起着主要作用.电生物体系中存在着电极与细胞色素c和脱氢酶的辅酶（NAD）及污染物间的逆向电子传递途径,可实现电极-微生物-污染物多相界间的长程电子传递.

英文摘要：

The reductive degradation of dichlorophenol（2,4-DCP） was studied in an electro-biological system （electric-assisted microbial system）. The colony structure was analyzed by polymerase chain reaction-single strand conformational polymorphism（PCR-SSCP） and a clone library. The results show that the dichlorophenol d phylogenetic tree was constructed by 16S rRNA gene egradation in the electro-biological system conforms to the kinetics characteristics of zero-order reaction. Enterococcus, Pseudomonas and Bacteroidales are the domi- nant bacteria and play a major role in the electron transfer for the electro-biological system. The reverse elec- tron transfer exists between the electrode, cytochrome c, NAD and the pollutants. A long-range electron trans- fer process of the muhiphase interfaces is achieved between the electrode, bacteria and pollutants.