中文摘要：

目的探讨Ghrelin和Obestatin对正常大鼠空间学习记忆能力的影响。方法大鼠随机分为Ghre-lin高剂量组、低剂量组和对照组-1;Obestatin高剂量组、低剂量组和对照组-2。Ghrelin各组双侧海马CA3区微量注射不同剂量Ghrelin和等量生理盐水;Obestatin各组侧脑室微量注射不同剂量的Obestatin和等量生理盐水。应用水迷宫方法检测大鼠的空间学习记忆能力。结果水迷宫隐匿平台实验中Ghrelin高剂量组的潜伏期与对照组-1相比明显缩短,撤平台实验中Ghrelin高剂量组在原象限游泳的时间和总路程的百分比与对照组相比明显增加。Obestatin各组在隐匿平台和撤平台实验中的结果都没有统计学差异。结论海马微量注射Ghrelin可明显提高大鼠的空间学习记忆能力,该作用可能与Ghrelin激活海马部位有关;侧脑室注射Obestatin,在水迷宫实验中未能观察到大鼠空间学习记忆能力的提高。

英文摘要：

Objective To explore the effects of Ghrelin and Obestatin on the ability of learning and memory in rats. Methods Rats were randomly divided into six groups as： high-dose Ghrelin （HDG）, low-dose Ghrelin （LDG） and control-1 ; highdose Obestatin （HDO）, low-dose Obestatin （LDO） and control-2. Different doses of Ghrelin and same amount of normal saline were given by micro-injection into the hippocampus CA3 in Ghrelin group; Different doses of Obestatin and same amount of normal saline were given by lateral cerebroventricular injection （icy）. The ability of learning and memory was tested by Morris water maze. Results The latency of swimming time and distance in the HDG were decreased significantly compared with that in the control group. In the probe test, the rats in HDG stayed longer time and swimming distance in the origin quadrant were longer than that of the control group. The results showed no difference between Obestatin groups and control group in the hiding platform test and probe test. Conclusion Micro-injection of Ghrelin into the hippocampus has an effect of improving the ability of learning and memory in rats, whereas icy injection of Obestatin has no such effect.