中文摘要：

目的 探讨核黄素在兔和人尸体眼球巩膜组织内达到饱和的时间,以指导巩膜紫外光（蓝光）-核黄素交联法中合理用药.方法 实验研究.取人尸体眼球5只,将每只眼的巩膜切成7个巩膜条带,分别给予0.1％核黄素溶液浸润5、10、15、20、25、30 min,行冰冻切片后在共聚焦显微镜下观察成像,计算巩膜条的荧光强度,并对浸润不同时间核黄素的各组进行比较.另选择新西兰白兔36只,采用SPSS软件中的随机数字生成器随机分为两组（每组18只）,右眼分别给予0.1％和0.5％核黄素溶液浸润,左眼作为对照.核黄素浸润后5、10、15、20、25、30 min各处死3只兔取眼球行鼻上象限赤道部巩膜的冰冻切片,在双光子显微镜下观察成像,计算巩膜条的荧光强度.采用单因素方差分析对尸体眼和同组内兔眼巩膜组织核黄素浸润后不同时间的荧光强度进行比较;采用t检验对同一时间点两组兔巩膜组织荧光强度进行比较.结果 应用0.1％核黄素溶液5、10、15、20、25、30 min后,人尸体眼球巩膜内核黄素的平均荧光强度分别为1.73±0.58、4.36±2.01、5.77±1.82、7.38±2.34、8.34±2.30、8.69±2.27.应用0.1％核黄素溶液5、10、15、20、25、30 min后,兔眼球巩膜内核黄素的平均荧光强度分别为6.54±0.65、9.38±0.34、11.31±111、15.97±2.54、17.46±1.78、18.49±2.78;应用0.5％核黄素溶液5、10、15、20、25、30 min后,兔眼球巩膜内核黄素的平均荧光强度分别为9.32±1.15、14.25±0.40、19.57±3.33、25.42±1.88、25.59±1.54、26.16±1.77.随着核黄素应用时间的延长,巩膜内的荧光强度逐渐增强,而20 min后各组间荧光强度差异无统计学意义（人尸体眼F=0.60,P＞0.05,兔眼0.1％核黄素F=0.84,P＞0.05,兔眼0.5％核黄素F=0.15,P＞0.05）;对于浸润相同时间核黄素的兔眼巩膜,0.5％浓度组的荧光强度高于0.1％浓度组（t=-3.65、-16.26、-4.08、-5.19、-5.99、-4.03;P＜0.05）.结论 核黄素

英文摘要：

Objective To investigate the permeability and saturation characteristics of riboflavin in sclera tissue,in order to explore a reasonable dosage regimen in sclera cross-linking.Methods Experimental study.Five human cadaver eyes were also included in this study,every eye was cut into seven sclera stripes and then 0.1% riboflavin solution was dripped to the sclera stripes for 5,10,15,20,25,30minutes respectively.After that,the stripes were made into frozen sections and observed by confocal microscope.To calculate the mean fluorescence density and compare with each other.In addition,36 New Zealand rabbits were randomly divided into group A and B groups.Each group was divided into six subgroups.In group A,0.1% riboflavin solution was dripped to the sclera of right eye for 5,10,15,20,25 and 30 minutes respectively.In group B,0.5% riboflavin solution was dripped to the sclera of right eye for 5,10,15,20,25 and 30 minutes respectively.The left eye were given with physiological saline as blank check.Executed the rabbits to take out the eye and then the nose upper quadrant of the equator sclera was taken to made into frozen sections which were observed by two-photon microscope.To calculate the mean fluorescence density and compare the six subgroups in every group respectively,and compare the subgroups with the same riboflavin-applicated time between group A and B respectively.Results The fluorescence intensity of sclera gradually strengthened with the longer riboflavin-applicated time,but there was no statistical difference between 20 minutes and the longer time groups （P〉0.05）.With the same riboflavin-applicated time in rabbit sclera,the fluorescence intensity of 0.5% concentration group was higher than 0.1 % concentration group （P〈0.05）.Conclusions The riboflavin reached an saturation level in the sclera of human cadaver eyes and rabbit eyes after 20 minutes application.The higher concentration of riboflavin-applicated,the higher concentration of riboflavin in the rabbit sclera.