中文摘要：

罗伊氏乳杆菌的MRS培养基是富培养基,简化培养基组分,优化培养基组成和培养条件是微生物工业化培养的重要基础。本实验在传统MRS培养基基础上,首先对氮源进行单因素优化,然后采用Plackett-Burman和中心组合试验设计对影响罗伊氏乳杆菌CG001生长的MRS培养基和培养条件进行筛选优化,并在最优条件下研究该菌的生长代谢情况。结果表明：酵母膏质量浓度、葡萄糖质量浓度、硫酸锰质量浓度和温度是影响菌体质量浓度的4个关键因素;经响应面法分析确定该菌的最优培养条件为：酵母膏质量浓度20g/L,葡萄糖质量浓度20g/L,醋酸钠质量浓度7g/L,柠檬酸铵质量浓度1g/L,磷酸氢二钾质量浓度3g/L,硫酸镁质量浓度0.2g/L,硫酸锰质量浓度为0.23g/L,吐温-80质量浓度1g/L,初始pH6.2,培养温度38.6℃,最终菌体质量浓度达0.984g/L,相对优化前提高1.102倍。发酵罐中菌体生长曲线呈S型,4～12h菌体处于对数生长期,之后趋于平衡,葡萄糖的代谢与菌体的生长速率相对应。

英文摘要：

MRS medium for Lactobacillus reuteri is an enrichment medium. It is essential for industrial fermentation to simplify medium composition and optimize selected constituents and culture conditions. Based on traditional MRS medium, nitrogen sources were optimized through single factor experiments. Plackett-Burman and central composite designs were then used to optimize medium and cultureconditions for exploring the growth of L. reuteri CG001. Finally, the growth metabolism of L. reuteri CG001 was investigated under optimized conditions. Results indicated that the concentrations of yeast extract, glucose, manganese sulfate and culture temperature were the most important factors affecting the growth of L. reuteri CG001. According to response surface analysis, the optimal conditions were 20 g/L yeast extract, 20 g/L glucose, 7 g/L sodium acetate, 1 g/L ammonium citrate, 3 g/L K2HPO4, 0.2 g/L magnesium sulfate, 0.23 g/L manganese sulfate, 1 g/L Tween-80, initial pH 6.2, and culture temperature of 38.6 ℃. The final cell amount reached up to 0.984 g/L under the optimal conditions, which exhibited 1.102-fold improvement. An ＂S＂-shaped pattern of the growth curve for L. reuteri CG001 in fermentor was observed. Cell growth was at the logarithmic phase during the period of 4 - 12 h, followed by a balance state between glucose metabolism and cell growth.