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Co-culture with microglia promotes neural stem cells differentiation into astrocytes

ISSN号：0366-6999
期刊名称：Chinese Medical Journal
时间：2011.10.10
页码：3394-3398
分类：Q421[生物学—神经生物学;生物学—生理学] Q813.11[生物学—生物工程]
作者机构：[1]Department of Breast PathologyTianjin Medical University Cancer Institute and Hospital, Key Laboratory of Breast Cancer Prevention and Therapy of the Ministry of Education and Key Laboratory of Cancer Prevention and Therapy of Tianjin, Tianjin 300060, China, [2]Department of Oncology , Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Breast Cancer Prevention and Therapy of the Ministry of Education and Key Laboratory of Cancer Prevention and Therapy of Tianjin, Tianjin 300060, China, [3]Department of Gynecology, Tianjin Central Gynecology and Obstetrics Hosoital, Tianiin 300052, China
相关基金：This study was supported by grants from China ＂973＂ Program （No. 2009CB521705）, ＂863＂ Program （No. 2006AA02A249 and No. 2007AA021802）, National Scientific Foundation of China （No. 30700253 and No. 30800355）, Key Program of National Scientific Foundation of China （No. 30930038）, China Program of Changjiang Scholar and Innovative Research Team in University （No. IRT0743）.
相关项目：乳腺浸润性微乳头状癌生长侵袭转移机制的分子基础

作者： Gu Feng|Wang Juan|Fu Li|Ma Yong-jie|

关键词：神经干细胞, 小胶质细胞, 细胞分化, 共培养, 星形胶质细胞, 中枢神经系统, 基因表达, 发展中国家, microglia, stem cells, astrogliogenesis

中文摘要：

背景神经干细胞( NSC )是一自我更新并且中央神经系统( CNS )的 multipotent 人口，它在开发期间活跃并且在整个 life.Microglias 维持动态平衡和织物完整是在 CNS 的一个有免疫力的房间人口居民，它在发展和学习试图调查那的成年 CNS.This 有关键生理的函数与 NSC co有教养的 microglia 是否能从 NSCs.Methods Microglia 和 NSC 支持 astrogliogenesis ，是 co-cultu

英文摘要：

Background Neural stem cells （NSCs） are a self-renewing and multipotent population of the central nervous system （CNS）, which are active during development and maintain homeostasis and tissue integrity throughout life. Microglias are an immune cell population resident in the CNS, which have crucial physiological functions in the developing and adult CNS. This study aimed to investigate that whether microglia co-cultured with NSCs could promote astrogliogenesis from NSCs. Methods Microglia and NSCs were co-cultured in 24-well insert plates. NSCs were plated in the bottom of the well and microglia in the insert. Fluorescent staining, Western blotting and RT-PCR were used to determine the effect of microglia on NSCs differentiation. Results Co-culture of microglia and NSCs promoted astrogliogenesis from NSCs. Several key genes, such as Notch 1, Notch 2, Notch 3, Hes 5, and NRSFwere downregulated, while the critical genes Idl and Id2 were upregulated. BMP2 and FGF2 were upregulated. Conclusion Microglias act as a regulator of NSCs astrogliogenesis.

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