中文摘要：

利用LSM 510 META激光扫描共聚焦显微成像系统,提出了一种能够获得胶原蛋白和弹性蛋白高对比度成像的双通道连续探测成像技术,并应用于食道基质层中的胶原蛋白和弹性蛋白的成像研究。实验结果表明,两个独立通道的使用显著地提高了胶原蛋白和弹性蛋白成像的对比度。由于成像过程中收集的二次谐波和双光子激发荧光信号均是背向,为双通道连续探测成像技术有望推广到病理诊断等临床应用中提供了可能。

英文摘要：

A novel imaging method which can acquire high-contrast image of collagen and elastin,two-channel sequential detection imaging based on two-photon excited autofluorescence and second harmonic generation of biological tissues,is presented by using a Zeiss LSM 510 META laser scanning microscopy system.And this method is successfully used to obtain high-contrast image of collagen and elastin in the stromal layer of esophageal tissue at 84μm and 150μm depths.The experimental findings show that the use of two independent channels obviously increases imaging contrast of collagen and elastin.Both two-photon excited autofluorescence and second harmonic signals of tissue samples are collected in a backscattering geometry.This makes two-channel sequential detection imaging technique have a clinic application potential for pathological diagnosis.