中文摘要：

目的：观察转染miR-214抑制剂的口腔鳞癌SCC15细胞增殖、迁移、侵袭能力的变化，并探讨其机制。方法取体外培养的口腔鳞癌SCC15细胞，分为观察组、阴性对照组和空白对照组，观察组和阴性对照组分别用Lipofectamine2000转染miR-214抑制物、无关序列，空白对照组不转染。采用实时荧光定量PCR法检测转染24 h时各组细胞中miR-214的表达，用CCK-8法观察转染24、48、72 h各组细胞增殖情况，用划痕实验观察转染24 h时各组细胞迁移能力，用Transwell实验观察转染24 h时各组侵袭能力，用Western blotting法检测转染24 h时各组细胞中的Wnt通路拮抗因子Dickkopf相关蛋白-3（DKK-3）及Wnt通路关键基因β-catenin蛋白。结果转染24 h时，与阴性对照组和空白对照组相比，观察组细胞 miR-214相对表达量低，划痕愈合百分比低，穿膜细胞数少，β-catenin蛋白相对表达量低，DKK-3蛋白相对表达量高（P均＜0．05）。转染48、72 h观察组OD值低于阴性对照组和空白对照组（P均＜0．05）。结论转染miR-214抑制物的口腔鳞癌SCC15细胞增殖、迁移及侵袭能力下降。其机制可能是因为转染miR-214抑制物能抑制Wnt／β-catenin信号通路相关蛋白DKK-3、β-catenin蛋白表达。

英文摘要：

Objective To investigate the changes in proliferation,migration and invasion abilities of oral squamous-cell carcinoma SCC15 cells transfected by miR-214 inhibitor,and to explore the mechanism.Methods The oral squa-mous-cell carcinoma SCC15 cells cultured in vitro were randomly divided into the observation group,negative control group and blank control group.SCC15 cells in the observation group and negative control group were transfected with miR-214 in-hibitor and inhibitor-NC in vitro by using Lipofectamine2000,respectively,and SCC15 cells in the blank control group were not given any intervention.The real-time PCR was used to detect the expression of miR-214 at 24 h after transfection. CCK-8 assay was used to observe the proliferation of the cells at 24,48 and 72 h after transfection.Scratch test and Tran-swell assay were used to observe the migration and invasion abilities of the cells at 24 h after transfection.Western blotting was used to explore the expression of Dikkopf-related protein-3 DKK-3 andβ-catenin protein at 24 h after transfection.Re-sults At 24 h after transfection,compared with the negative control group and blank control group,the expression of miR-214 and β-catenin protein and wound healing rate was significantly lower,the number of penetrating cells was significantly less and the expression of DKK-3 protein was significantly higher in the observation group （all P〈0.05）.The A450 in the observation group was lower than that in the negative control group and blank control group at 48 and 72 h after transfection （all P〈0.05 ）.Conclusion The proliferation,migration and invasion abilities of oral squamous-cell carcinoma SCC15 cells transfected with miR-214 inhibitor decrease,and the mechanism may be that the transfection of miR-214 inhibitor can inhibit the expression of DKK-3 andβ-catenin protein in Wnt/β-catenin signaling pathway.