中文摘要：

建立同时检测菊花中7种化合物HPLC方法。采用乙腈-0.1%磷酸水溶液梯度洗脱,流速：0.8mL/min,进样量：10μL,检测波长：348nm,柱温：30℃,同时检测绿原酸、木犀草苷、3,5-0-二咖啡酰基奎宁酸、芦丁、黄芩苷、槲皮素和金合欢素等7种化合物。HPLC法图谱比较,发现6种不同菊花7种化合物成分峰面积比例具有很大差异。试验结果表明,该方法能够简便、灵敏、直观地揭示不同菊花的差异,为菊花的指纹图谱建立和菊花多成分的含量测定提供参考方法。

英文摘要：

To establish a method for determining 7 Kinds of Compounds in Flos Chrysanthemi,acetonitrile-0.1% phosphoric acid aqueous gradient elution,flow rate：0.8mL/min,sampling volume：10μL,detection wavelength：348nm,column temperature 30℃,simultaneous determination of caffeotannic acid,galuteolin,3,5-0-bis caffeoylquinic acid,rutin,baicalin,meletin and acacetin from seven Kinds of Compounds are used.Comparison of HPLC chromatograms has the founding that the peak area ratio of six different chrysanthemum from seven kinds of compound ingredients are very different.The results show that the method is simple,sensitive,direct-viewing,and reveal the differences of chrysanthemum.This provids a reference method for simultaneous determination of multicomponent in chrysanthemum flos and the establishment of fingerprint.