中文摘要：

肿瘤微脉管系统对肿瘤生长重要，转移并且这样肿瘤处理结果。交替的冷却并且加热的处理被证实了优于冷却或加热的单个处理。微脉管系统损坏由替换物导致了使位于下面的治疗和机制凉下来并且加热的肿瘤的度在这份报纸被学习。到包括交替的冷却并且加热的不同治疗的肿瘤微脉管系统的反应用裸体老鼠通过共焦的显微镜学在 vivo 被观察背面的皮褶层肿瘤房间模型。结果证明交替的冷却并且加热在整个全部肿瘤导致了更多严重损坏到 microvessel 结构。肿瘤容器墙上的机械压力的数字模拟在交替的处理期间发现了那，容器墙连续地在相反的方向在热压力承受快速的 chang，力量引起了它损坏到外部微脉管系统并且在中央容器微裂缝。在结冰以后的血流动的灌注也在容器墙上导致了相对大的压力，特别当血流动在随后的加热期间快速重新酒时。血容器上的压力的快增加可能是引起指试验性的观察的血容器破裂的关键问题之一。初步的学习部分揭示了位于微脉管系统损坏由使治疗凉下来并且加热的替换物引起了的严重肿瘤下面的机制。

英文摘要：

Tumor microvasculature is important to tumor growth, metastasis and thus tumor treatment outcome. The altemate cooling and heating treatment has been confirmed to have advantages over single treatment of cooling or heating. The degree of tumor microvasculature damage induced by the altemate cooling and heating treatment and the mechanisms underlying are studied in this paper. The response of the tumor microvasculature to different treatments including alternate cooling and heating is observed in vivo through confocal microscopy using the nude mice dorsal skin fold tumor chamber model. Results show that alternate cooling and heating has induced much more severe damage to the microvessel structure throughout the entire tumor. Numerical simulations of the mechanical stresses on the tumor vessel wall has found that during the alternate treatment, the vessel wall suffer a rapid chang in thermal stresses in the opposite directions successively, which might caused damage to the peripheral microvasculature and micro-cracks in the central vessels. Reperfusion of blood flow after freezing also led to relatively large stresses on the vessel wall, especially when blood flow re-perfuses quickly during the subsequent heating. The quick increase of stresses on the blood vessel might be one of the key issues causing the blood vessel rupture referring to the experimental observation. The preliminary study has partly revealed the mechanism underlying serious tumor microvasculatare damage caused by the alternate cooling and heating treatment.