加倍的 haploid (DH ) 人口在庄稼改进和基本研究对科学家和 breeders 有用。生产 DH 的当前的方法通常为为染色体加倍提取 haploids 和化学处理在 vitro 需要文化。这份报告为综合描述一个简单方法特别为由利用 meiotic 归还基因的 allopolyploid 种类的 DH (SynDH ) 。方法包含三个步骤:杂交将导致再结合,提取 haploids 的种间的杂交,和由 selfing 加倍种间的 F1s 的自发的染色体。这样生产的 DH 在对一个同类的背景的兴趣的染色体包含 recombinant 染色体。没有特殊设备或处理涉及 DH 生产,它能容易在任何繁殖或基因节目被使用。Triticum turgidum L。并且 Aegilops tauschii Coss,普通小麦(Triticum aestivum L.) 的二祖先的种和分子的标记被用来表明 SynDH 方法。
Doubled haploid (DH) populations are useful to scientists and breeders in both crop improvement and basic research. Current methods of producing DHs usually need in vitro culture for extracting haploids and chemical treatment for chromosome doubling. This report describes a simple method for synthesizing DHs (SynDH) especially for allopolyploid species by utilizing meiotic restitution genes. The method involves three steps: hybridization to induce recombination, interspecific hybridization to extract haploids, and spontaneous chromosome doubling by selfing the interspecific Fis. DHs produced in this way contain recombinant chromosomes in the genome(s) of interest in a homogeneous background. No special equipment or treatments are involved in the DH production and it can be easily applied in any breeding and/or genetic program. Triticum turgidum L. and Aegilops tauschii Coss, the two ancestral species of common wheat (Triticurn aestivum L.) and molecular markers were used to demonstrate the SynDH method.