中文摘要：

目的研究氢醌对体外培养条件下MOLT4细胞的毒性作用及细胞色素P450CYP4F3基因表达的意义。方法流式细胞术ANNEXINV-FITC加碘化丙啶（PI）双染定量检测细胞凋亡率和坏死率的变化;RT-PCR方法检测CYP4F3在mRNA表达水平,比较不同处理组之间的差异。结果加入不同浓度氢醌培养0、4、8、12、16 h后,流式细胞术检测结果发现,不同浓度氢醌作用后,细胞凋亡率明显高于空白对照组,差异有显著性（P〈0.01）,氢醌诱导细胞凋亡的最佳时间是8h,而随着作用时间延长,氢醌浓度为200μmol/L时,细胞坏死率明显增高;另外发现氢醌浓度为120μmol/L时,细胞凋亡率随着时间的延长逐步增加,8 h达到高峰,随后开始下降;CYP4F3基因在mRNA表达水平与细胞凋亡率一致。结论体外培养条件下,CYP4F3基因的表达上调可能对氢醌诱导MOLT4细胞凋亡起促进作用,并存在一定量-效与时-效关系。

英文摘要：

Objective To study the cytotoxic effect induced by hydroquinone in MOLT4 cells in vitro,and the significance of cytochromo P450CYP4F3 expression.Methods Apoptotic and necrotic rate were examined by flow cytometer with Anti-AnnexinV /FITC Plus PI staining.The mRNA expression of CYP4F3 was detected by RT-PCR,and the differences in differently treated groups were compared.Results After adding different concentrations of hydroquinone to the cells for 0,4,8,12,16 h culture,the cell apoptotic rate in different concentrations of hydroquinone groups was significantly higher than that in blank control groups（P〈0.01）.The optimal time induced by hydroquinone was 8 h.With time extension,the necrotic rate increased significantly at the concentration of 200 μmol /l.The apoptosis induced by hydroquinone was associated with culture time at the concentration of 120 μmol /l,and the peak apoptotic time was 8 h,then the apoptotic rate decreased.Furthermore,the amount of CYP4F3 expression at the mRNA level was in accordance with the apoptosis rate.Conclusions Hydroquinone could induce apoptosis of MOLT4 cells,the up-regulation of CYP4F3 expression probably promoted hydroquinone to induce MOLT4 cells to go into apoptosis in vitro with dose-effect and timeeffect relationship.