从福建龙海红树林区土壤中分离获得9株产脂肪酶的细菌,经16SrDNA序列分析表明分别属于红球菌属(Rhodococcus)、库克菌属(Kocuria)、假单胞菌属(Pseudomonas)、芽胞杆菌属(Bacillus)和微小杆菌属(Exiguobacterium).对9株细菌所产的脂肪酶进行酶学性质研究,结果显示这些酶的最适作用温度在25~35℃、最适作用pH值在8~10,其中L13菌株所产脂肪酶(L’13)具有适应温度和pH范围较广、对多种金属离子耐受性强、能有效降解不同底物等特点,在环境保护和工业生产方面具有良好的应用前景.
9 strains lipase-producing bacteria from mangrove soil of Longhai, Fujian were isolated and characterized. The results of 16S rDNA sequence analysis showed that those bacteria belonged to Rhodococcus,Kocuria,Pseudornonas, Bacillus and Exiguobacterium. Preliminary studies on properties of lipase products from those bacteria demonstrated that the optimal temperature was 25℃ to 35℃ ,the optimal pH was 8 to 10. The lipases (L'13) produced by L13 had an effective role in a wide range of temperature and pH. They were also resisted m a variety of metal ions and could effectively degrade different substrates. Due to these properties,we suppose the lapses will have a broad application prospect in industrial production and environmental protection.