以11种华山松挥发物单体和2种不同受害状态的华山松树皮挥发油为材料,利用嗅觉仪测定华山松大小蠹对不同浓度的不同挥发物的行为反应。结果表明:华山松大小蠹雌、雄成虫对(S)-(—)-β-蒎烯表现出趋避反应,而对10种挥发物质单体和不同被害阶段华山松挥发油均表现出趋向反应;在低浓度刺激下,华山松大小蠹雌、雄成虫对α-菲兰烯表现出显著的趋向反应;随着浓度的升高,华山松大小蠹雌性成虫对(S)-(—)-α-蒎烯、(—)-莰烯和α-菲兰烯也表现出明显的趋向反应。而华山松大小蠹雄性成虫则对(R)-(+)-α-蒎烯和3-蒈烯表现出明显的趋向反应;雌虫对α-菲兰烯的趋向反应显著高于(R)-(+)-α-蒎烯、(S)-(—)-α-蒎烯、(—)-莰烯和3-蒈烯,而雄虫对α-菲兰烯和(R)-(+)-α-蒎烯的趋向反应显著大于对(S)-(—)-α-蒎烯、(—)-莰烯和3-蒈烯的反应。同时华山松大小蠹雌虫对(—)-莰烯、健康华山松韧皮部挥发油和被害华山松韧皮部挥发油的行为反应显著大于雄虫。华山松大小蠹对一些华山松挥发物的行为反应具有性别差异性,也对不同种类和浓度挥发物的行为反应存在差异。
Behavior responses of laboratory adults of Dendroctonus armandi were tested to 11major monoterpenes and volatile compounds from phloem of Pinus armandi by using a Y-tube olfactometer.The results showed that besides the avoidance effect of(S)-(β)-β-pinene on D.armandi,other ten monoterpenes tested could evoke approach behavior responses of male and female D.armandi at different concentrations,includingα-phellandrene,(S)-(—)-β-pinene,(R)-(+)-α-pinene,(S)-(—)-α-pinene,(+)-camphene,(—)-camphene,(R)-(+)-limonene,(S)-(—)-limonene,3-carene,β-myrcene,bornyl acetate and two volatile compounds from healthy and attacked host trees.Male and female bark beetles only showed approach behavior response toα-phellandrene when tested at a low concentration of volatile stimuli;male bark beetles showed approach response toα-phellandrene,(R)-(+)-α-pinene and 3-carene,while females showed approach response to(S)-(—)-α-pinene,α-phellandrene and(—)-camphene at a concentration level of 100μg/μL;males showed more significant approach response toα-phellandrene and(R)-(+)-α-pinene than to(S)-(—)-α-pinene,(—)-camphene and 3-carene,whereas females were more sensitive toα-phellandrene than to(R)-(+)-α-pinene,(S)-(—)-α-pinene,(—)-camphene and 3-carene.There existedsex differences in behavioral responses of the D.armandi to some volatiles of Chinese white pine and D.armandi showed different behavioral responses to different volatiles at different concentration levels.