中文摘要：

根据Pib基因的结构特点,分别构建了不同启动子驱动Pib基因ORF的表达载体pNAR701、pNAR704和pNAR705,分别由35S＋Pib启动子、35S启动子和Pib启动子驱动;采用农杆菌介导转化水稻品种日本晴。经PCR、Southern blot分析证实了Pib基因已经稳定整合到日本晴的基因组中;Northern blot、实时荧光定量PCR对Pib基因表达分析表明：pNAR701转基因后代株系中目的基因的表达量较pNAR704和pNAR705高,且同一载体的不同转基因后代株系间存在着表达量的差异。对T1、T2代苗期稻瘟病抗性鉴定显示：不同启动子驱动的Pib结构基因mRNA的表达,都表现出对稻瘟菌生理小种ZB1和ZG1的高抗特性,但不同启动子驱动的该基因编码区mRNA的表达与稻瘟病抗性水平间没有明显差异。

英文摘要：

According to the structure of Pib gene,we built different expression vectors named pNAR701,pNAR704 and pNAR705,respectively,and each drove the ORF of Pib gene by promoter of 35S ＋ Pib,35S and Pib.All constructions were done with Agrobacterium-mediated transformation into Nipponbare,and the results of PCR and Southern blot analysis confirmed that the Pib gene fragment has been inserted into the genome of Nipponbare.It showed that the expression of expected gene in pNAR701 transgenic lines was higher than that in pNAR704 and pNAR705,and the same transgenic event in different transgenic lines was various in expression of target gene by Northern blot,Real-time PCR and rice blast resistance analysis.In addition,the identification of blast resistance of T1 and T2 in seedling showed that 35S ＋ Pib promoter,35S promoter and endogenous promoter of Pib gene which drove the code region of Pib gene displayed high resistance to race ZB1 and ZG1,and there was no obvious difference between each other in resistance.