中文摘要：

【目的】为揭示生防菌吡咯伯克霍尔德氏菌JK-SH007进入自然环境后对周围的微生态因子是否存在威胁,对其进入自然环境后的微生态效应和生物安全性做出正确评估。【方法】采用固体平板法研究JK-SH007菌株对美洲黑杨盆栽苗土壤微生物种群数量的影响,对4种主要土壤酶（酸性磷酸酶、碱性磷酸酶、脱氢酶、转化酶）的活力进行测定,并通过Biolog ECO微孔板法分析JK-SH007菌株对美洲黑杨土壤功能多样性的影响。【结果】从整体趋势来看,在美洲黑杨根际引入JK-SH007菌株后,随着时间延长,根际土壤中的细菌、放线菌、真菌数量逐渐高于对照,且差异显著,在接种后150天接种处理达到峰值（3.36×10^9cfu·g^-1干土;7.50×10^7cfu·g^-1干土;1.14×10^7cfu·g^-1干土）,随后显著降低,但仍优于未接种处理;JK-SH007处理后土壤酶活性均有所增强,土壤酸性磷酸酶、碱性磷酸酶、脱氢酶、转化酶活性均大于对照,其中,接种对碱性磷酸酶、转化酶活性的促进最为明显;根际土壤中微生物的AWCD值（除了在接种后30,90天时）,接种处理和未接种处理的AWCD值相当,几乎持平;而接种后10,20,60,120,150,180天时接种处理土样微生物的AWCD值均高于CK;土壤微生物多样性（Shannon指数、Simpson指数和Mc Intosh指数）在接种前期没有明显的规律性,与对照几乎持平,120,150,180天时,接种JK-SH007处理的Simpson指数、Shannon指数、Mc Intosh指数均高于对照,但差异不显著。【结论】在根际引入JK-SH007对美洲黑杨根际土壤微生物的整体活性和功能多样性的提高有一定的促进作用,在一定程度上丰富了土壤微生物种群,有利于保持和促进土壤肥力和健康状况,但这些影响随着时间的延长而减弱;同时也说明JK-SH007菌株的施入不会对环境中土著微生物构成威胁或威胁较小,符合Bcc菌的安全应用范围,进一步证明该菌株的生物安全性,为将来

英文摘要：

【Objective】Burkholderia pyrrocinia JK-SH007 is a strain of biocontrol bacteria which was isolated from the stem of poplar. In order to evaluate the safety of B. pyrrocinia JK-SH007,this study aimed to evaluate the micro ecological effects and the biological safety of the natural environment after its entry into the natural environment.【Method】A solid plate method was used to investigate the potential effects of JK-SH007 on the activity change of the four main soil enzymes,and the Biolog ECO microplate method was applied to assay the microbial community and its function diversity in rhizosphere soil of aigeiros poplar（ Populus deltoides）. 【Result】The results showed that from the over trend,the numbers of bacteria,actinomyces and fungi in poplar rhizosphere soil gradually increased and were significantly higher than control with the extension of time after being inoculated with strain JK-SH007. The number of these microorganisms reached the peak（ 3. 36 × 10^9cfu·g^-1dry soil; 7. 50 × 10^7cfu·g^-1 dry soil; 1. 14 × 10^7cfu·g^-1dry soil） in 150 day post-inoculation,and then decreased significantly,but by then were still higher than these of control plants. The soilenzymes activities（ acid phosphatase,alkaline phosphatase,dehydrogease and invertase） with inoculation of strain JKSH007 were all higher than that of control,especially for alkaline phosphatase and invertase. The Biolog ECO plates analysis showed that AWCD values on day 10,day 20,day 60,day 150 and day 180 after inoculation were higher than control treatment. At the early stage of the inoculation,soil microbial diversity（ Shannon index,Simpson index and Mc Intosh index） had no obvious regularity. At the 120 th day,150 th day and 180 th day post-inoculation,the three parameters of the functional diversity of the soil microbial communities were all slightly higher than those of control. But,the differences were not significant. 【Conclusion】Strain JK-SH007 may play roles in improving total microbial activity of poplar rhiz