应用AMMI模型、线性回归模型和系统聚类分析方法分析了9个水稻光温敏核不育系和2个对照不育系(培矮64S,浙农大11S),在9个播期环境下花粉和种子育性的变化动态。从AMMI两维图可直观看到基因型与播期的交互模式,根据最大互作效应主成分轴IPCA1值和花粉育性平均值将不育系分成3个集团。第1集团是培矮64S(1)、浙大21S(10)和浙大22S(11),具有较低花粉育性平均值和较高IPCAl值,表明它们对温度变化比较敏感,且有较大的负向互作效应。第2集团是浙农大11S(2)、浙大4S(3)、浙大5S(4)、浙大7S(6)、浙大8s(7)和浙大9s(8),其花粉育性平均值和互作值相对较低,变动在0.013~0.276间,暗示花粉育性对播期敏感度低。第3集团是浙大6S(5)和浙大10S(9),花粉育性平均值高,互作效应大,该2个不育系尚有分离,且花粉育性对播期反映敏感度高。对光敏型和温敏型不育系而言,基因型IPCA值大小主要反映它们对光周期和温度敏感性强弱。花粉和种子育性在基因型、播期及其互作效应上都存在极显著差异。本文还提出利用育性相对稳定性的定量指标Di界定光温敏核不育系的育性稳定性,分析表明,Di值与育种实践结果较为接近。基于AMMI模型的基因型主效应和互作效应分析可以明确划分不育系的不育期、育性转换期和可育期,并将水稻光敏型与温敏型不育系区分开,因而该模型可为不育系应用于种子生产提供信息和依据。
Pollen and seed fertility data of nine P (T) GMS and the two control sterile lines(Pei' ai 64S,ZAUllS) were analyzed based on AMMI model, linear regression model and hierarchical cluster analysis under the nine sowing dates. The Biplot of this AMMI analysis showed clearly the interactive pattern between the genotypes and the sowing dates. According to their IPCA1 value and mean pollen fertility, the male-sterile lines could be divided into three groups. The first group [ Pei'ai 64S(1), ZU21S (10) and ZU22S (11) ] had the lower mean pollen fertility and the higher IPCA1 (the largest Interaction Principle Components Axis) value, indicating quietly sensitive to the temperature change, and the great negative effect. The second group which involved ZAU11S (2), ZU4S (3), ZUSS (4), ZU7S (6), ZU8S (7) and ZU9S (8), displayed relatively lower pollen fertility mean and considerably lower IPCA1 value with a range of 0.013 -0.276, suggesting their lower sensitivity of the pollen fertility to the different sowing dates. The fertilities of lines ZU6S (5) and ZU10S (9) listed in the third group were segregated still, and with the higher values of mean pollen fertility and the interactive effect. For the PGMS and the TGMS lines, the IPCA value of genotypes mainly reflects their susceptibilities levels to photopefiod and temperature. There were different significantly in the pollen and seed fertility for genotypes, sowing date and their interaction. The results of main effects and interaction effects analysis were different between the two parameters. The parameter Di ,was proposed as an index for measuring the fertility stability of P (T) GMS rice in this paper. The research results indicated the estimates of Di were feasible and greatly concordant with the breeding practice. The sterility stage,fertility alteration stage and fertility stage as well as the photo-sensitive sterile lines and the thermo-sensitive sterile lines in rice could be distinguished by the AMMI