采用基物培养、燕麦-琼脂培养技术及扫描电镜技术对灰团网菌的个体发育过程进行了研究,在燕麦琼脂培养基上完成了从孢子到孢子的生活史.结果表明:灰团网菌生活史包括单核的黏变形体或游动胞、多核的营养体原质团以及孢子形成阶段.孢子球形,表面具疣突;孢子萌发为孔式,一个孢子可释放一个黏变形体.黏变形体常行变形运动,在有水的条件下,可转变为游动胞并具有游动的特性.合子形成原质团.成熟原质团白色,类型为显型,具有扇形网络状菌脉.琼脂培养基上获得的灰团网菌孢子与野生型相似,并具有可育性.
The life history of myxomycetes is of importance to study diversity of nutritive modes and phylogenesis of these organisms.Until now the reports concered were few.Moist chamber culture method,oat-agar culture method and scanning electron microscopy were used to study the process of the individual development of Arcyria cinerea.The life cycle of spore-to-spore were completed in agar culture in petridish.The result shows that the life cycle of Arcyria cinerea comprises a unicellular amoeboid or swarm cell stage,a multinucleate plasmodium stage and a sporulation stage.The spore of Arcyria cinerea is globular and the spore surface is spiny.The spore germinates by means of a poriform open and releases a single myxamoeba.The myxamoeba can move by amoeboid motion.When free water is available,myxamoebae can transform into swarm cells and swim in the water.Zygote forms into plasmodium.The mature plasmodium is white.The plasmodial type is the phaneroplasmodium which appears as a fan-shaped network of veins.Spores developed on agar are fertile and resemble those on natural substrate.