中文摘要：

将4个转录因子Oct4，Sox2，Klf4和c—Myc转入成纤维细胞可以生成诱导多能干细胞（iPS细胞），转录因子和染色质修饰因子在这个过程中起重要作用。LSD1作为染色质结构的调节因子，在早期胚胎发育和Es细胞分化中发挥着关键作用。为了探索LSD1在iPS细胞产生过程中的作用，首先比较了LSD1蛋白在MEFs和ES细胞中的表达量，然后分别通过在重编程体系中过表达LSD1、加入RNAi和抑制剂的方法探索LSD1的功能，最后用免疫共沉淀的方法初步发现LSD1的作用机制。结果表明，LSD1在Es细胞中的表达量高于MEFs中，过表达LSD1对iPS细胞的形成效率没有影响，而RNAi抑制LSD1的表达和LSD1抑制剂tranylcypromine都能促进iPS细胞的形成。免疫共沉淀实验表明LSD1和Oct4／Nanog有相互作用。这些数据说明LSD1通过和Oct4／Nanog相互作用调控iPS细胞的形成。

英文摘要：

Fibroblasts can be reprogrammed to induced pluripotent stem （iPS） cells upon expression of Oct4, Sox2, Klf4 and c-Myc. Transcription factors and chromatin modifiers are important to this reprogramming process. As one of the chromatin regulators, LSD1 plays critical roles in early embryogenesis and ES cell differentiation. Westem blot analysis showed that the expression level of LSD1 in ES cells is higher than in MEFs. Overexpression of LSD1 in the reprogramming system has no effect on the generation of iPS cells. However, more iPS cell colonies are formed when the expression of LSD1 is knockdown by RNAi or with LSD1 inhibitor tranylcypromine. Coimmunoprecipitation showed the physical interaction between LSD1 and Oet4/Nanog. In all, these data suggests that LSD1 regulates the generation of iPS cells via interaction with Oet4/Nanog.