中文摘要：

目的探讨海马微环境的变化对体外培养的放射状胶质细胞增殖和分化的影响。方法本实验在成功制备正常侧和切割穹隆海马伞侧海马提取液的基础上，分别使用正常侧和切割侧海马提取液，观察其在体外培养的放射状胶质细胞增殖和分化过程中所起的作用。结果在放射状胶质细胞增殖阶段，切割组与正常组及对照组相比，5-溴脱氧尿嘧啶核苷（BrdU）标记的增殖细胞比例明显提高（P〈0．05）；在放射状胶质细胞分化阶段，切割组与正常组及对照组相比，Tujl阳性神经元数量明显增多（P〈0．05）。结论切割穹隆海马伞侧海马提取液对大鼠放射状胶质细胞的增殖和向神经元的分化有明显促进作用，提示海马微环境对海马放射状胶质细胞的增殖和分化有重要影响。

英文摘要：

Objective To investigate the proliferation and differentiation effect of hippocampal niche on the radial glia cells in vitro. Methods Successfully prepared normal hippocampal extracts and transected extracts after hippocampal fimbria-fornix transection. On the proliferation and differentiation stages, the normal and transected extracts were used to investigate their effects on the radial glia cells respectively. Results On the proliferation stage, the proportion of BrdU- positive cells in the transected group was obviously higher than that of the normal group and control group（P 〈 0.05 ） ; on the differentiation stage, the number of Tujl-positive cells in the transected group was much more than that of the normal and control group （ P 〈 0. 05 ）. Conclusion The fimbria-fornix transected hippocampal extracts can promote the proliferation and neurons differentiation of neonatal rats radial glia cells in vitro. These data indicate that the hippocampal niche is a pivotal effecting factor associated with proliferation and differentiation of the radial glia cells.