中文摘要：

目的：探索微RNA（miRNA，miR-）-let-7e-3p在不同宫颈病变中表达情况及临床意义。方法：收集并整理具有完整临床病理资料的病例，其中正常子宫颈26例、子宫颈高级别鳞状上皮内病变（ HSIL）37例、宫颈癌101例；采用实时定量RT-PCR检测临床宫颈组织样本中miR-let-7e-3p的相对表达量，分析miR-let-7e-3p表达水平与宫颈癌的发生及临床不良预后因素间的相关性。将miR-let-7e-3p模拟物转染至宫颈癌细胞株Siha（转染组），采用流式细胞仪检测Siha细胞周期和细胞凋亡变化；CCK-8试剂盒检测Siha细胞增殖变化；Transwell实验检测Siha细胞迁移和侵袭能力变化。结果：miR-let-7e-3p在正常宫颈、HSIL及宫颈癌组织中的相对表达量分别为1．45±0．24、0．79±0．05、0．46±0．04，正常子宫颈与 HSIL、正常子宫颈与宫颈癌、HSIL 与宫颈癌相对表达量差异均具有统计学意义（均P＜0．05）；miR-let-7e-3p表达水平与患者宫颈癌临床不良预后因素之间无显著相关性；转染组 S 期细胞（29．76％±6．60％）高于阴性对照组（13．38％±1．30％，P＜0．05）；转染组凋亡率（5．98％±1．38％）高于阴性对照组（3．53％±0．79％，P＜0．05）；转染组转染后48、72、96 h 吸光度值分别为0．57±0．11、0．65±0．04、0．84±0．14，阴性对照组为0．74±0．05、0．93±0．10、1．47±0．14，细胞增殖均被抑制（均P＜0．05）；转染组与阴性对照组迁移和侵袭能力差异均无统计学意义（均P＞0．05）。结论：miR-let-7e-3p可能在宫颈癌的发生中发挥抑癌基因的作用，阻滞细胞周期和抑制细胞增殖可能是其重要调控机制。

英文摘要：

Objective：To investigate the expression of microRNA （ miRNA, miR） let-7e-3p in different cervical lesions and its clinical significance.Methods： The expression of miR-let-7 e-3 p in the tissues of normal cervix （ n =26 ） , high-grade squamous intraepithelial lesion （HSIL） （n =37）, and cervix carcinoma （n =101） were detected by reverse transcription and quantitative polymerase chain reaction （RT-qPCR）.The correlation of miR-let-7e-3p expression with the clinicopathological parameters of patients with cervical cancer was analyzed.miR-let-7e-3p mimic was transfected into cervical carcinoma Siha cells. The cell cycle and apoptosis were determined by flow cytometry;cell proliferation was determined by CCK-8 kit;and the migration and invasion of cells were determined by Transwell assay.Results： The relative expression levels of miR-let-7e-3p in normal cervix, HSIL, and cervical carcinoma were 1.45 ±0.24, 0.79 ±0.05 and 0.46 ±0.04, respectively （ all P 〈0.05） .After transfection with miR-let-7e-3p mimic, the S-phase fraction and apoptosis rate of Siha cells were increased significantly compared with control group [（29.76 ± 6.6）%vs （13.38 ±1.3）%, P〈0.05; （5.98 ±1.38）% vs （3.53 ±0.79）%, P〈0.05, respectively].OD of transfected Siha cells at 48, 72 and 96 h were 0.57 ±0.11, 0.65 ±0.04 and 0.84 ±0.14, which were significantly lower than those of untransfected Siha cells （0.74 ±0.05, 0.93 ±0.10 and 1.47 ±0.14, all P〈0.05）.The migration and invasion abilities of transfected Siha cells were not significantly changed （ all P〉0.05）.Conclusion： The expression of miR-let-7e-3p is down-regulated in cervical neoplasms, which is associated with cell cycle arrest and proliferation inhibition of cervical cancer cells.