中文摘要：

利用芹菜胚性细胞悬浮系成功分离得到大量原生质体,获得芹菜大量原生质体的最佳反应体系为：酶液组成为3.0%纤维素酶Onozuka R-10、1.0%离析酶R-10、11%甘露醇、0.5%CaCl2·2H2O和0.1%MES;摇床转速为80r/min,温度（25±2）℃,酶解时间5～6h;原生质体产量为25.00×10^6/g,原生质体活力83.41%。原生质体浅层培养,培养基为1/2MS＋1mg/L2,4-D＋0.5mg/LKT＋11%甘露醇＋500mg/L水解络蛋白,两天后,重新再生细胞壁之后进行第1次分裂,逐步降低渗透压至甘露醇3%,大约30d形成小细胞团。小愈伤组织经增殖培养后在1/2MS＋500mg/LCH＋0.25mg/LKT固体分化培养基诱导出不定芽,30d后再转入MS基本培养基,获得完整的再生植株。

英文摘要：

Protoplasts were successfully isolated from embryogenic suspensions incubating in enzyme solution containing 3.0% cellulose Onozuka R-10, 1.0% macerozyme R-10, 0. 5% CaCl2· 2H2O, 0. 1% MES and 11% mannitol. The enzyme mixture was shaken （80 r/min） for 6 h at （25 ±2）℃. The yield and viability were 25. 00 ×10^6/g and 83.41%. Purified protoplasts were cultured in mediem 1/2 MS ＋ 1 mg/L 2, 4-D ＋0. 5 mg/L KT ＋ 11% mannitol ＋500 mg/L CH initially with shallow liquid layers. Two days later, cell wall was regenerated and simultaneously intiated the first division, through reducing manntol, mini-culli were observed in 30 days. Shoots regenerated in medium 1/2 MS ＋0. 25 mg/L KT ＋ 500 mg/L CH.