中文摘要：

目的：探讨累加电针治疗神经源性痛大鼠的作用机制。方法：Wistar大鼠随机分为正常对照组，慢性压迫性损伤（CCI）组，CCI＋EA2（天）D组，CCI＋EA2（周）w组，去卵巢（OVX）＋CCI组，OVX＋CCI＋EA2D组，OVX＋CCI＋EA2W组。去卵巢大鼠在手术后每天颈部皮下注射D一半乳糖，连续45天造成神经记忆损伤。结扎坐骨神经造成慢性神经疼痛模型，电针双侧“足三里”一“阳陵泉”穴1次／D，分别电针2D、2W。用RT—PCR的方法检测下丘脑前阿黑皮素（POMC）mRNA和海马、下丘脑孤啡肽前体（PPPNOC）mRNA的表达。结果：与正常对照组比，CCI组下丘脑POMCmR．NA表达水平显著升高（P〈0．05），海马及下丘脑PPNOCmRNA表达水平也显著升高（P〈0．05）。与CCI组比，CCI＋EA2W组POMCmRNA表达水平进一步显著上调（P〈0．05），而PPNOCmRNA表达水平显著降低（P〈0．05）。在OVX＋CCI动物上，与OVX＋CCI组比，OVX＋CCI＋EA2W组下丘脑POMC的表达量明显上调（P〈0．05），而OVX＋CCI＋EA2D和OVX＋CCI＋EA2W海马PPPNOCmRNA的表达显著下调（P〈0．05），但后两组下丘脑PPPNOCmRNA的表达无显著变化（P〉0．05）。结论：电针对慢性神经痛大鼠的累积性镇痛效应与下丘脑POMCmRNA表达的上调和海马PONCmRNA表达的下调密切相关；记忆损伤可减弱电针上调下丘脑POMC基因的表达。

英文摘要：

Objective：To investigate the mechanism of repeated electroacupuncture （EA） underlying the cumulative analgesic effect in neuropathie pain rats. Methods ： A total of 35 Wistar rats were randomized into control （ n = 5 ） , chronic constrictive injury （CCI） （n=5）,CCI＋EA2day （D） （n=5）,ovaficctomy （OVX） ＋CCI （n=5）,OVX＋CCI＋EA2D （n=5）,OVX＋CCI＋ EA2 weeks （W） （ n = 5） groups. The OVX rats were subcutaneously injected with D - galaetose （15mg/100g） solution, once daily for 45 days （D）. Chronic pain model was established by ligating the left sciatic nerve with pieces of surgical suture. EA（2/ 15Hz 1- 2mA ） was applied to bilateral ＂Zusanli＂ （ ST 36） - ＂Yanglingquan＂ （ GB 34） for 30 min, once daily for 2D and 2 W, respectively. Hypothalamic Pre - proopiomelanocortin （ POMC ） mRNA expression, and hypothalamic and hippocampal prepronociceptin （PNOC） mRNA expression were detected by reverse transcription polymerase chain reaction （ RT - PCR）. Results：In comparison with the normal control group, the hypothalamic POMC mRNA expression was upregulated significantly （ P 〈 0. 05 ） and hypothalamus and hippocampal PPNOC mRNA expression downregulated considerably in CCI group （ P 〈 0. 05 ）. Compared with the CCI group, the expression of hypothalamic POMC mRNA was increased markedly in CCI ＋ EA2W group （P 〈 0. 05 ）, while the expression of hippocampal PNOC mRNA decreased distinctly in both CCI ＋ EA2D and CCI ＋ EA2W groups （P 〈 0. 05）. In OVX ＋ CCI rats, compared with the OVX ＋ CCI group, hypothalamic POMC mRNA expression was upregulated significantly in OVX ＋ CCI ＋ EA2W group （ P 〈 0. 05 ）, whereas hippocampal PNOC mRNA expression was downregulated remarkably （ P 〈 0.05）. No significant differences were found between the CCI and CCI ＋ EA2D groups, and between the OVX ＋ CCI and OVX ＋ CCI ＋ EA2D groups in the expression of hypothalamic POMC mRNA;between the CCI ＋ EA2D and CCI ＋