中文摘要：

农产品细胞内的ATP含量水平直接反映细胞的活性,在产后贮藏过程中可作为农产品新鲜度和品质的一种评价指标。采用紫外-可见-近红外分光光度计UV-3600获取菠菜叶片样本的光谱数据,并采用常规物理化学方法和萤火虫荧光素酶生物发光技术制备和测定细胞原生质体悬浮液及其细胞原生质体的ATP含量。利用person相关分析确定关键特征波长,并建立基于298nm紫外光和730nm近红外光两个特征波长菠菜叶片光谱反射率的细胞原生质体ATP含量的预测模型。结果分析表明,298nm紫外光和730nm近红外光两个特征波长具有预测细胞原生质体ATP含量的潜力（R2=0.802 9和0.901）。提出的基于光谱技术的蔬菜细胞ATP含量检测方法为准确、快速、无损的蔬菜新鲜度评价提供一种新的技术途径。

英文摘要：

The cellular ATP content level in agricultural products directly reflects cell viability, therefore it can be potentially used as an indicator of freshness and quality of agricultural products during storage. Spectral data of sample spinach leaves were obtained using a UV-Vis-NIR spectrophotometer UV-3600. Protoplast suspensions were prepared by following the conventional physical-chemical methods, and the ATP contents in protoplasts were determined by the firefly luciferase bioluminescence technology. Person＇s correlation analysis was performed to identify the key wavelengths. Models were developed for estimating the ATP contents in spinach protoplasts based on the two identified key wavelengths, i.e. the ultraviolet 298 nm and the near-infrared 730 nm wavelengths. Results showed that both of the two key wavelengths （298 and 730 nm） have a considerable promise in estimating the ATP content in spinach protoplasts （R2=0. 802 9 and 0. 901 respectively）. The spectroscopy based estimation of cellular ATP content in vegetables proposed in this study provides a new approach to the accurate, rapid, and non-destructive evaluation of the freshness of vegetables.