中文摘要：

目的分析不同病期感染兔血清所识别的日本血吸虫成虫抗原（adult worm antigen，AWA）和可溶性虫卵抗原（soluble egg antigen，SEA）的组分蛋白，探索可能与Th1／Th2极化相关的主要功能性抗原。方法常规方法制备成虫和虫卵抗原，用SDS-PAGE电泳和Western blotting技术，分析鉴定感染动物血清抗体识别的虫源性及卵源性组分抗原。结果不同病期血清抗体识别的AWA、SEA组分蛋白谱不同。其中AwA中的11kDa、37kDa、57kDa、79kDa、95kDaa组分分子能被各病期（2～20w）感染血清抗体所识别；SEA中的10kDa、18kDa、41kDa、47kDa、74kDa则可被急性期及以后各病期（6～20w）血清抗体所识别，25kDa、32kDa仅为急性期（6～12w）血清抗体所识别，提示这些组分抗原可能为血吸虫免疫应答的主要功能性抗原，并且同感染血清的反应性明显与病期相关。结论以上AwA和SEA的组分蛋白和不同病期感染血清的反应与血吸虫感染后的Th1／Th2免疫偏移相关，其中能被急性期及以后各病期血清抗体共同识别的SEA主要功能性组分抗原中可能存在诱导Th2极化反应的重要抗原分子。

英文摘要：

In order to investigate the key functional antigen related to Th1/Th2 polarization by identifing fractional antigens from adult worms and eggs with sera of rabbits infected with Schistosoma japonicum in different stages of disease, both adult worm and egg antigens were prepared and the SDS-PAGE and Western blotting were used to analyze and identify the dif- ferent fractions of antigens with sera in different stage. The results showed that 11 kDa,37 kDa,57 kDa,79 kDa,95 kDa fractions of adult worms were recognized serum antibodies taken 6-20 weeks after infection; while 10 kDa, 18 kDa, 41 kDa, 47 kDa, 74 kDa fractions of egg antigens were recognized serum antibodies,taken 6-20 weeks after infection 25 kDa, 32 kDa were recognized by serum antibodies taken 6-20 weeks after infection. These findings suggest that these functional proteins were the key functional antigens, which may be related to infection stage and Th2 polarization in hosts infected with Schistosoma japonicum.