中文摘要：

目的探讨多房棘球绦虫（Em）重组BCG-EmⅡ／3疫苗免疫和Em原头节攻击后小鼠脾细胞因子的变化。方法将Balb／c小鼠按体质量随机分为3组：疫苗皮下注射组、疫苗鼻腔内接种组、对照组。免疫后8周用Em原头节进行攻击感染，感染后18周杀鼠取脾，分离脾细胞，分别用原液、Em抗原（EmAg）、刀豆蛋白A（ConA）或植物血凝素（PHA）培养，酶联免疫吸附（ELISA）法检测脾细胞培养上清液中白细胞介素2（IL-2）、1干扰素（IFN-γ）、肿瘤坏死因子α（TNF-α）、白细胞介素4（IL-4）水平。结果疫苗皮下注射组原液培养条件下IL-2、IFN-γ、TNF-α和IL-4水平分别为（34．6±2．7）、（34．5±2．8）、（265．0±0．0）、（9．8±2．6）ng／L，与对照组[（25．0±1．9）、（30．0±0．0）、（10．0±0．0）、（12．5±2．7）ng／L]比较差异均有统计学意义（P〈0．01或〈0．05）；疫苗鼻腔内接种组原液培养条件下上述4种指标分别为（32．5±2．2）、（33．6±2．7）、（130．0±0．0）、（10．4±2．7）ng／L，与对照组比较差异均有统计学意义（P〈0．01或〈0．05）；疫苗皮下注射组原液培养条件下TNF-α水平明显高于疫苗鼻腔内接种组（P〈0．01）。EmAg、ConA（或PHA）刺激培养条件下，上述4种指标明显高于相同组别的原液培养（P〈0．01），且ConA（或PHA）刺激培养条件下，上述4种指标明显高于相同组别的EmAg刺激培养条件（P〈0．01）。结论多房棘球绦虫重组BCG－EmⅡ／3疫苗诱导小鼠产生辅助T淋巴细胞（Th）1型免疫反应，从而对抗Em原头节攻击感染。

英文摘要：

Objective To investigate the changes of cytokines of splenocytes in mice immunized with recombinant BCG-Em Ⅱ/3 vaccine of Echinococcus multilocularis（Em）and consequently ,challenged with Em protoscoleces. Methods Balb/c mice were subcutaneously or intranasally vaccinated and challenged with Em protoscoleces on the 8^th week of vaccination. After they were killed on the 18^th week of infection, their splenocytes were separated and cultured with EmAg, ConA or PHA, respectively. The supernatants were gathered to measure the levels of IL-2, IFN-γ, TNF-α and IL-4 by ELISA Kits. Results The levels of IL-2, IFN-γ, TNF-α and IL-4 in the subcutaneous group were（34.6 ± 2.7）, （34.5 ± 2.8）, （265.0 ± 0.0） and （9.8 ± 2.6）ng/L respectively; those in the intranasal group were （32.5 ± 2.2）, （33.6 ± 2.7）, （130.0 ± 0.0） and （10.4 ±2.7）ng/L respectively; those in the control were （25.0 ± 1.9）,（30.0 ±0.0）,（10.0 ±0.0） and （12.5 ± 2.7）ng/L, respectively; there were statistical differences between the immunized groups and control group（P 〈 0.01 or 〈 0.05 ） ; The level of TNF-α in the subcutaneous group was higher than that in the intranasal group. Conclusion Th1 response has been induced in mice vaccinated with rBCG-Em Ⅱ/3 vaccine and challenged with Em protoscoleces.