中文摘要：

目的：观察脾阳虚大鼠小肠黏膜上皮细胞形态学变化，探讨脾阳虚证胃肠功能改变的组织病理学基础，并观察温阳健脾代表方理中汤的治疗作用。方法：复合因素合利血平注射法建立脾阳虚大鼠模型；通过小肠黏膜组织石蜡切片HE染色光镜检查、透射电镜观察等方法，对造模及治疗后大鼠小肠黏膜进行组织病理学观察。结果：光镜观察未见脾阳虚大鼠小肠黏膜上皮细胞有明显病理学改变；但透射电镜下小肠黏膜上皮细胞超微结构可见明显改变：上皮细胞间隙紧密连接变宽；粗面内质网间隙增宽；线粒体肿胀，嵴断裂、减少或结构模糊，甚至空泡化。理中汤治疗后上述病变有明显改善。结论：观察到脾阳虚大鼠小肠上皮细胞内细胞器的形态改变，从一个侧面反映脾阳虚证组织病理学基础；理中汤对脾阳虚证的治疗作用机制可能与改善上述表现有关。

英文摘要：

Objective： To study the histopathology and ultramicrostructure changes on spleen-yang deficiency rat model and explore the histopathology of the dysfunction of spleen and stomach of spleen yang deficiency. Method： The composite factors method was used to set up the spleen yang deficiency rat model. Then reablement were given to model rats with or without Li-zhong decoction for 4 weeks. The small intestine was observed with light microscope or transmission electron microscope separately. Results： There was no obviously histomorphology difference between normal and spleen yang deficiency rats under light microscope. But obviously organelle changes were found inside those epithelial cells under transmission electron microscope in spleen yang deficiency group. In Li-zhong decoction group, the epithelial cells＇ organelle changes were not found so obviously. Conclusion： Changes on morphous and structure of spleen-yang deficiency rats＇ intestinal mucosa under transmission microscopy may be one of the histopathology reasons of dysfunction of nutrients absorption of small intestine and Li-zhong decoction may cure that in this way.