中文摘要：

小麦抗秆锈病基因Sr33对强毒力小种Ug99和我国多数小麦秆锈菌小种具有良好抗性。以感病品种McNair701和来源于TetraCanthatchlTriticumtauschii的单基因系Sr33为亲本，选用我国流行的小种34MKG,对作图群体161个F2单株及其F2：3家系进行抗性鉴定分析，利用分离群体集群分析法对位于小麦1D染色体上的57对微卫星引物进行扩增多态性筛选。获得2对在亲本及F2抗、感群体间揭示多态性的共显性标记Xbarcl52和Xcfdl5，位于Sr33两侧，与目标基因的遗传距离分别为2．4cM和2．1cM。

英文摘要：

Gene Sr33 confers resistance not only to Ug99, but also to most Puccinia graminis f. sp. tritici races in China. A map- ping population, consisting of 161 F2 plants and F2：3 families derived from the cross between susceptible wheat cultivar ＂McNair701＂ and a Tetra Canthatch/Triticum tauschii line carrying single Sr gene Sr33, were inoculated with Chinese prevalent race 34MKG to locate this resistance gene using microsatellite markers. A total of 57 microsatellite markers covering chromosome 1D were used in bulked segregation analysis （BSA） based on resistance identification at seedlings stage. Two codominant markers, Xbarc152 and Xcfdl5, were obtained, which were located at both sides of Sr33. The genetic distances of Xbarc152 and Xcfdl5 to the target gene were 2.4 cM and 2.1 cM, respectively. These closely linked markers can be used in marker-assisted selection of wheat breeding agalnst stem rust disease.