中文摘要：

目的：探讨阴离子交换蛋白在人视网膜色素上皮（RPE）细胞非特异性吞噬过程中的作用。方法：体外培养人RPE细胞，以荧光包被的乳胶株作为吞噬标记物，建立RPE细胞吞噬模型。使用不同浓度阴离子交换蛋白阻滞剂DIDS（1、10、100、200、300、500和1000μmol·L^-1）预处理RPE细胞，37℃孵育6h，利用流式细胞术定量检测RPE细胞对乳胶珠的吞噬指数。结果：经6h孵育，未加DIDS的对照组RPE细胞对乳胶株的吞噬指数为（35．1±1．6）％，加入1μmol·L^-1 DIDS的RPE细胞吞噬指数为（34．1±2．1）％（P〉0．05）．加入10μmol·L^-1 DIDS的RPE细胞吞噬指数为（28．7±1．9）％，10μmol·L^-1的DIDS显著地抑制了RPE细胞对乳胶株的吞噬作用（P〈0．05），并且随着DIDS浓度的增加（100、200、300、500及1000μmol·L^-1）．RPE细胞吞噬指数逐渐降低[吞噬指数分别为（23．0±1．2）％、（17．0±1．5）％、（12．0±1．6）％、（7．0±2．2％）及（5．0±2．5）％]，与对照组比较差异均有显著性（P〈0．01）。结论：抑制阴离子交换蛋白的活性将导致人RPE细胞非特异性吞噬功能的障碍，阴离子交换蛋白可能参与人RPE细胞的非特异性吞噬过程。

英文摘要：

Objective To investigate the role of anion exchange in nonspecific phagocytic process of human retinal pigment epithelial （RPE） cells. Methods Fluorescent latex beads were incubated with RPE cells at 37℃, flow cytometry was performed on RPE cells that had been challenged with fluorescent latex beads. The quantity of phagocytosis was expressed as a phagocytic index. Experiments were also performed in the presence of different concentration anion exchange blocker DIDS （1, 10, 100, 200, 300, 500 and 1 000 μmol·L^-1）. Results Phagocytic index of RPE cells was （35.1±1.6）% at 6 h of incubation without DIDS. Phagocytic index of RPE cells was （34.1±2.1） % in the presence of 1μmol·L^-1 DIDS （P〉0.05）. Phagocytic index of RPE cells was （28.7±1.9） % in the presence of 10μmol·L^-1 DIDS, 10μmol·L^-1 DIDS significantly inhibited the nonspecific phagocytic process of human RPE cells （P〈0.05）, and DIDS inhibited the process in concentration-dependent manner. Phagocytic index of RPE cells was （23.0±1.2）%, （17.9±1.5）%, （12.0±1.6） %, （7.0±2.2）% and （5.0 ± 2.5）% in the presence of 100, 200, 300, 500 and 1 000 μmol·L^-1 DIDS （P 〈 0.01）. Conclusion The inhibition of anion exchange activity will result in the decrease of nonspecific phagocytosis in RPE cells, anion exchange maybe participate in the nonspecific phagocytic process of RPE cells.