中文摘要：

目的探讨芩丹胶囊（QC）含药血清对动脉血管外膜成纤维细胞（AF）的作用机制。方法采用组织贴块法体外原代培养大鼠胸主动脉成纤维细胞,MTT法观察大[750mg/（kg·d）]、小[150mg/（kg·d）]剂量的QC含药血清对转化生长因子β1（TGF-β1）诱导的大鼠胸主动脉成纤维细胞的增殖的影响;实时定量PCR及western-blot分别检测α-平滑肌肌动蛋白（α-SMactin）和Smad3的基因及蛋白表达情况。结果大、小剂量QC含药血清呈剂量依赖性抑制TGF-β1诱导的AF的增殖（P〈0.05）,且用药组α-SMactin和Smad3基因及蛋白的表达较单纯刺激组显著降低（P〈0.05）。结论QC含药血清通过干扰TGF-β1/Smad信号转导通路抑制AF增殖和分化,从而改善和逆转动脉血管外膜重构。

英文摘要：

Objective To investigate the effect and mechanism of the Qindan Capsule-containing serum on adventitial fibroblasts（AFs）. Methods AFs were cultured by tissue explant in vitro and induced by TGF-β1. The proliferation of AFs affected by the Qindan Capsule-containing serum with high[750 mg /（kg·d）]or low[150 mg /（kg·d）]dose was detected by MTT. Protein and mRNA expressions of α-SM actin and Smad3 were observed by Western-blot and Real-time PCR respectively. Results Both high and low doses of the Qindan Capsule-containing serum could reduce proliferation of AFs induced by TGF-β1（P〈0. 05） and inhibit expressions of mRNA and protein of α-SM actin and Smad3（P〈0. 05）. Conclusion The Qindan Capsule-containing serum has significant improved and reverse effect on aortic vascular remodeling. The mechanism may be related to inhibiting proliferation and differentiation of AFs through the TGF-β1 /Smad signaling pathway.