中文摘要：

目的：应用肿瘤坏死因子α（TNF-α）诱导3T3-L1脂肪细胞,探讨建立可靠胰岛素抵抗（IR）细胞模型的方法。方法：3T3-L1前脂肪细胞经3-异丁基-1-甲基黄嘌呤、地塞米松、胰岛素诱导分化成3T3-L1脂肪细胞,将其与20,10,5μg.L-1TNF-α共孵育,100 nmol.L-1胰岛素作用30 min刺激脂肪细胞糖转运。以葡萄糖氧化酶法测定培养基上清液葡萄糖含量,观察TNF-α对脂肪细胞糖摄取的影响,鉴定IR模型。结果：TNF-α抑制胰岛素诱导前、后的脂肪细胞糖转运,抑制作用呈剂量依赖性,其中20μg.L-1TNF-α的抑制率分别为79.2%和81.4%（P〈0.05）。结论：肿瘤坏死因子α可诱导3T3-L1脂肪细胞产生IR,这种细胞模型简便、可靠。

英文摘要：

Objective：To study the effect of tumor necrosis factor α（TNF-α） on glucose consumption in 3T3-L1 adipocyte and find the method of establishing a cell insulin resistance model.Method：The 3T3-L1 preadipocytes were differentiated to 3T3-L1 adipocytes with 1-methyl-3-isobuthylxanthine,dexamethasone and insulin.3T3-L1 adipocytes were exposed to 20,10,5 μg · L-1 TNF-α for 96 h,then added 100 nmol · L-1 insulin for 30 min.The amount of glucose consumption was determined by detecting the glucose content in cell culture supernatants with glucose oxidase（GOD） assay.Result： TNF-α markedly decreased both basal and insulin-stimulated glucose uptake.This effect was concentration-dependent,and 20 μg · L-1TNF-α decreased glucose uptake of 79.2% and 81.4%（P〈0.05） in the basal and 100 μg · L-1 insulin-stimulated conditions,respectively.Conclusion：20 μg · L-1TNF-α can induce insulin resistance in 3T3-L1 adipocytes.A novel and reliable cell model of insulin resistance is successfully established.