中文摘要：

大黄素（emodin）是从大黄中提取的一种天然蒽醌类衍生物,具有明显的抗肿瘤活性。研究大黄素对人鼻咽癌细胞株CNE增殖的抑制作用以及其诱导凋亡的机制,对其抗肿瘤机制的研究具有积极的意义。利用MTT法检测大黄素对人鼻咽癌细胞株CNE增殖抑制作用,通过Hoechst33342染色,观察大黄素处理人鼻咽癌细胞株的形态学变化,Western blot研究其诱导肿瘤细胞凋亡通路的变化。结果表明,对于试验细胞株,大黄素杀伤肿瘤细胞的作用,随大黄素浓度升高和作用时间的增加,呈剂量依赖性和时间依赖性;通过倒置显微镜观察细胞在给药后的直观形态学变化,给药后通过Hoechst33342染色进一步证明大黄素可诱导凋亡。给药后通过Western blot对其作用机制的初步探索表明,大黄素处理后聚腺苷二磷酸核糖聚合酶（PARP）发生剪切,增强Caspase依赖性细胞凋亡通路的信号。大黄素单药可以抑制人鼻咽癌细胞CNE的增殖,表明其有可能成为一种有效的治疗鼻咽癌的药物。

英文摘要：

Emodin（1,3,8-trihydroxy-6-methylanthraquinone） is an active component from the root and rhizome of Rheum palmatum that has been reported to exhibit antitumour effects.However,the molecular mechanisms of emodin-mediated apoptosis in human nasopharyngeal carcinoma cells have not been fully investigated.The study investigated the effects and mechanisms of emodin-induced cell death in human nasopharyngeal carcinoma cell line CNE.Cell viability was measured by MTT method.The cell morphology alteration was detected with Hoecst 33342 nuclear staining.Western blot was performed to measure the protein expression level.We demonstrated the critical role of emodin in the suppression of the proliferation of CNE cells based on a dose-and time-dependent manner.The IC50,determined after 48 h incubation,was 55.03±0.12μmol/L for CNE.CNE Cells were obeserved by inverted microscope to study the cell morphology directly.We found that emodin treatment induced remarkable apoptosis,evidenced by increased cleavage of PARP.Taken together,these results demonstrated that emodin induced CNE cells death via apoptosis,suggesting that emodin could be a promising therapeutic agent against nasopharyngeal carcinoma.