中文摘要：

背景：细胞膜片技术无须支架材料和酶消化,通过刺激细胞外基质分泌形成致密膜片组织,能有效地修复组织缺损和改善器官功能。目的技术和新进展,为相关基础和临床研究：回顾近年来国内外细胞膜片技术应用于组织工程领域的新提供新的思路。方法：由第一作者检索CNKI全文数据库、万方数据库及PubM ed数据库2010年1月至2015年7月的相关文献,检索关键词为＂细胞膜片,组织工程;cell sheet,tissue engineering＂。结果与结论：与传统细胞膜片技术相比,支架材料与细胞膜片技术相整合用于重建和修复坚韧组织器官,多层细胞膜片相互层叠形成三维组织修复软组织器官等新技术不断涌现。与单层细胞膜片相比,通过相同或不同的细胞片层叠而成的3D细胞膜片具有更强组织再生功能,而且在体外就能构建出理想的靶组织模型。利用支架材料复合细胞膜片技术来提高复合物机械强度和减少细胞流失,并用于牙齿、骨与软骨等机体坚韧组织修复取得较大进展。目前细胞膜片技术仍主要处于实验室研究阶段,临床应用研究不多,期待有更多的创新性手段融入到细胞膜片技术上来。

英文摘要：

BACKGROUND： The cell sheet technology that is applied with the absence of scaffolds and enzymatic digestion can effectively repair tissue defects and improve organ function, by stimulating the secretion of extracellular matrix to form a dense membrane tissue.OBJECTIVE： To review the recent progress in cell sheet technology used in tissue engineering, thereby providing a new idea for relevant basic and clinical research. METHODS： The first author retrieved CNKI database, Wanfang database and Pub Med with the keywords of ＂cell sheet, tissue engineering＂ in Chinese and English, respectively. Literature retrieval period was from January 2010 to July 2015. RESULTS AND CONCLUSION： Cell sheet technology combined with scaffold materials can be used for reconstruction and repair of tissues and organs. With the emerging of new technologies, multi-layer cell sheets are stratified to form a three-dimensional tissue for repair of soft tissues and organs. Compared with the monolayer cell sheet, the three-dimensional cell sheet that is laminated by same or different cell sheets has stronger regenerative ability and can be used to construct the ideal target tissue model in vitro. Cell sheet technology combined with scaffolds can improve the mechanical strength of the composite and reduce cell loss, which has made great progress in the repair of tooth, bone and cartilage tissue. Currently, the cell sheet technology is at the laboratory stage, and little is reported on its clinical applications. We look forward to more innovative technologies that can be integrated into the cell sheet technology.