中文摘要：

以温敏雄性不育系K121S在可育温度（10℃）下和不育温度（26℃）下三核期花蕾为材料,以可育温度下的cDNA为驱动子,通过抑制性差减杂交技术构建了不育温度下抑制性差减杂交（SSH）文库,其插入片段长度分布在200～1 000 bp左右,并随机挑选97个阳性菌落测序,得到20个表达序列标签（EST）,包括与ATP合酶β亚基、Rubp羧化酶小亚基同源的EST等,为进一步获得育性转换相关基因和揭示K121S的育性转换分子机理奠定了基础.

英文摘要：

In this study, using flower-bud at thinucleate stage from thermo-sensitive male-sterile line K121S （Brassica juncea） at fertile temperature and sterile temperature as material, SSH library was constructed with driver cDNA obtained at male-fertile temperature. Ninety-seven clones were chosen randomly in sequencing. Twenty EST were discovered analogous to genes such as ATP synthetase sub- unit beta, ribulose-1, 5-bisphosphate carboxylase small subunit. The size of the DNA inserted ranges from 200 to 1 000 bp. The library laid the foundation of obtaining of the genes for fertility conversion and clarification of molecular mechanism of K121S fertility conversion.