中文摘要：

目的观察亚慢性铅暴露小鼠睾丸组织铅蓄积和对小鼠精子质量影响，并探讨二者的关联性。方法昆明种小鼠60只，按体重将小鼠随机分为对照组、0．5/L、1．O/L和1．5/L醋酸铅染毒纽。通过自然饮用含不同浓度醋酸铅水的方式使小鼠染铅，连续染毒60d后取附睾和睾丸组织。用电感耦舍等离子体质谱仪（ICP—MS）检测小鼠睾丸组织的铅浓度，常规方法检测附睾精子质量，并用统计学方法对二者的相关性进行分析。结果0．5g／L、1．0/L和1．5g／L染铅组小鼠精子的活动度分别为87．3％、79．2％和68．3％，显著低于对照组（94．1％）（P〈0．05）、、各铅暴露小鼠精子畸形率分别为15．2％、32．8％和35．5％，显著高于对照组（7．0％）（P〈0．05）。各铅暴露小鼠睾丸组织铅浓度分别为72．71ng/g，118．74ng/g和177．14ng／g，显著高于对照组（9．73ng／g）（P〈0．05），并随染毒剂量增加而升高。而且，铅暴露小鼠睾丸铅浓度与精子的活动率、密度和存活率之间呈显著负相关（P〈0．05）。结论亚慢性铅暴露可导致小鼠睾丸组织铅蓄积，铅在睾丸组织蓄积可能是亚慢性暴露小鼠精子质量下降的重要因素。

英文摘要：

Objective To observe effect of subehronie exposure to lead （Pb） on sperm quality of mice and accumulation of Pb in the testis. Methods Sixty mice were divided into 4 groups of 15 each. Group 1 received drinking water alone （con- trol）. Group 2, 3 and 4 received 0. 5, 1.0 and 1.5 g/L Pb acetate respectively. Pb acetate was given through drinking wa- ter. On the 60th day after Pb exposure, mice were deeapitated and the epididymis and testis were taken. After the treat- ment, the concentration of Pb was determined in testis of mice by inductively coupled plasma - mass spectrometry （ ICP - MS） and the sperm quality was observed by microscope. The correlation between concentration of Pb in testis and toxic effect of it on the sperm quality was also analyzed by Linear regression analysis. Results The activity of sperm in mice was 9.73 ng/g in control group and 87.3% , 79.2% , amt 68.3% respectively in the 0.5, 1.0 and 1.5 g/L Pb acetate grot, ps. The aetivity of＇ sperm was significantly lower in the experimental groups than that in controls （ P 〈 O. 05 ）. The sperm malformation rate was 15.2c% , 32.8% and 35.5% in the groups exposed to Pb and significantly increased compared with control group （7.0%） （ P 〈 0. 05 ）. Concentration of Pb in the testis was 72.71, 118.74, and 177.14 ng/g respectively in the 3 experimental groups and significantly higher than that in control group （9.73 ng/g） （ P 〈0.05 ）. Moreover, there was significant negative correlation between the Pb eoncentration in testis and density, survival rate, and viability of sperm in mice exposed to Pb. Conclusion The subchronic exposure to Pb can induce the accumulation of Pb in the testis of mice. It indicates that the accumulated Pb in testis may be responsible for the decreased sperm quality in mice exposed to Pb.