中文摘要：

为了揭示甘蓝Ogura胞质雄性不育系发生线粒体同源异型转化的分子机制,利用RT-PCR和转基因的方法分别进行了研究。RT-PCR试验结果表明,甘蓝B基因AP3在雌蕊（心皮）发育初期异位高表达,C基因AG3在发生心皮化的雄蕊部位异位高表达,这可能导致了甘蓝Ogura胞质不育系线粒体同源转化的发生。而拟南芥转基因试验表明,线粒体定位表达载体p3301-ATPORF138能导致拟南芥雄性不育的发生,这说明orf138基因是发生线粒体同源异型转化的甘蓝Ogura胞质雄性不育系不育的决定因素。

英文摘要：

RT-PCR and transgenic method were used to elucidate the molecular mechanism of mitochondria homeosis in cabbage Ogura CMS. The RT-PCR research indicated that the high ectopic expression of homeobox gene B（AP3） was discovered in green pistil（carpel）,and which of C gene（AG3） was discovered in carpelloid stamen, which probably caused mitochondria homeosis in cabbage Ogura CMS. In addition,the test with transgenic Arabidopsis with mitochondria-directed expression vector p3301-ATP-ORF138 indicated that ORF138 could cause male sterile of Arabidopsis,which revealed that orf138 was a determinant factor of male sterility in cabbage Ogura CMS with mitochondria homeosis.