中文摘要：

目的了解缺氧新生大鼠脑组织中Sox10蛋白的表达。方法 40只3日龄新生SD大鼠,随机分为对照组和缺氧组。对照组新生大鼠不做任何处理;缺氧组新生大鼠置于密闭容器中,充以氧氮混合气体（80 mL.L-1氧气,920 mL.L-1氮气）90 min。分别于造模3 d和7 d后留取其脑组织,采用HE染色、免疫组织化学、Western blot检测脑损伤新生大鼠脑组织Sox10表达。采用SPSS11.5软件进行分析。结果缺氧后新生大鼠相继表现为烦躁不安、全身发绀,呼吸加深加快、站立不稳、嗜睡、激惹或间断发作的痉挛和抽搐;HE染色显示缺氧组大鼠大脑皮质出现局灶性神经元核固缩,核碎裂,核仁偏位、不清或消失,基质疏松;免疫组织化学及Western blot结果显示缺氧组新生大鼠脑组织Sox10表达较对照组明显增加,差异有统计学意义。结论缺氧可以造成新生大鼠脑损伤,Sox10在缺氧时明显表达,提示Sox10通过剂量的升高对缺氧新生大鼠脑组织进行保护性修复作用。

英文摘要：

Objective To investigate the expression of Sox10 protein in brain tissues of newborn rats with hypoxia.Methods Forty 3-day neonatal SD rats were randomly divided into control group and hypoxia group.No treatment was given in control group,but rats in hypoxia group were put in a closed vessel containing mixed gases（80 mL·L-1 oxygen and 920 mL·L-1 nitrogen） for 90 min.The protein expression of Sox10 in brain tissues of rats were determined with HE staining,immunohistochemistry and Western blot on the 3rd and 7th day,respectively after animal models were established.The data were analyzed with SPSS 11.5 software.Results Neonatal rats in hypoxia group manifested restlessness,cyanosis,deep and rapid breath,astasia,lethargy,irritation and spasm;there was focal pyknosis of neuron,fragmentation,dissymmetry,blur or disappearance of nucleus,and raritas of cellular matrix.The expression of Sox10 in brain tissues in hypoxia group were significantly higher than that in control group indicated by immunochemistry and Western blot,and there was significant difference between 2 groups.Conclusions Hypoxia can cause hypoxia-ischemia brain damage in premature rats.The expression of Sox10 is higher in brain tissues of hypoxia rats,which indicates that Sox 10 is up-regulated and plays its protective role in hypoxia brain.